[Histonet] Oil Red O

[Amos Brooks]

Hi,
     I have seen this too. I mitigate the problem by making it up in 50 ml
increments and staining them flat. I draw from around the middle of the
Falcon tube I make it up in. It tends to precipitate so you could filter
it. Don't bother re-using the reagent. It's cheap & easy to make up. I
usually only see the precipitate with old solutions. Just make what you use
within a few weeks.

Best 'o luck,
Amos



>
> Message: 2
> Date: Wed, 3 Oct 2018 15:26:25 +0000
> From: Reuel Cornelia <Reuel.Cornelia at tsrh.org>
> To: "histonet at lists.utsouthwestern.edu"
>         <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] Oil Red O
> Message-ID:
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>
> I was wondering if you could help me know why our Oil Red O have some
> black snowflakes on our fat tissue hours after staining or after 24 hours
> more snowlflakes precipitation occurs.  The staining was reference was from
> Lillie RD, Ashburn. Please note that our staining works well between two to
> three hours. Is there a reason fro this precipitation?
>
>
> Reuel
>
> TSRH
>
> Dallas, TX
>
>