[Histonet] Leica ST5050 immunostainer

Eddie Martin edmartin26 at gmail.com
Fri Mar 16 13:25:19 CDT 2018

Dr Fleming,

If you’re insistent on automation, I would suggest a used Leica Bond Max instead.  The Leica Bond Max is most likely the most cost effective, and has the smallest footprint while also minimizing reagent waste compared to a Dako Flex or Ventana XT/Ultra. 

If you are willing to do it by hand, there a few labs that I’m aware of that use Leica’s powervision  compact polymer system to stain their Melan-A’s. I’m sure it would stain a MITF just as easy. 

Eddie M. 

Team lead medical technologist
Histology Technical Supervisor
The National Institutes of Health 
Clinic Center
Bethesda, MD 20814
Eddie.martin at nih.gov

> On Mar 16, 2018, at 9:00 AM, Matthew Fleming <mgflem at gmail.com> wrote:
> Hi,
> I have a small dermatopathology lab and would like to introduce limited
> immunohistochemistry.  Initially I would probably just do MITF stains with
> red chromogen on a few cases per week. We could do this by hand but I've
> noticed a number of ST5050 immunostainers for sale at very low prices. I
> know they're quite old but we have a number of machines old or older that
> continue to serve us well. But so far I haven't been able to find a manual
> and am concerned about finding consumables. Leica apparently stopped
> supporting this model some time ago.  Is anyone out there still using it?
> Anyone have a manual? Anyone like to comment as to whether it might be
> usable for a limited IH operation?
> Thank you,
> Matthew Fleming, MD
> Fleming Dermatopathology
> Milwaukee, WI

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