[Histonet] Leica ST5050 immunostainer

[Jennifer Clark]

We are a small derm lab and have a Nemesis 3600 from Biocare.  It will
perform IHC on up to 36 slides per run and is an open platform.  We also do
a reagent rental through them.  The doctor is happy with the quality.

On Fri, Mar 16, 2018 at 2:25 PM, Eddie Martin via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Dr Fleming,
>
> If you’re insistent on automation, I would suggest a used Leica Bond Max
> instead.  The Leica Bond Max is most likely the most cost effective, and
> has the smallest footprint while also minimizing reagent waste compared to
> a Dako Flex or Ventana XT/Ultra.
>
> If you are willing to do it by hand, there a few labs that I’m aware of
> that use Leica’s powervision  compact polymer system to stain their
> Melan-A’s. I’m sure it would stain a MITF just as easy.
>
> Best,
> Eddie M.
>
> Team lead medical technologist
> Histology Technical Supervisor
> The National Institutes of Health
> Clinic Center
> Bethesda, MD 20814
> Eddie.martin at nih.gov
>
>
>
> > On Mar 16, 2018, at 9:00 AM, Matthew Fleming <mgflem at gmail.com> wrote:
> >
> > Hi,
> >
> > I have a small dermatopathology lab and would like to introduce limited
> > immunohistochemistry.  Initially I would probably just do MITF stains
> with
> > red chromogen on a few cases per week. We could do this by hand but I've
> > noticed a number of ST5050 immunostainers for sale at very low prices. I
> > know they're quite old but we have a number of machines old or older that
> > continue to serve us well. But so far I haven't been able to find a
> manual
> > and am concerned about finding consumables. Leica apparently stopped
> > supporting this model some time ago.  Is anyone out there still using it?
> > Anyone have a manual? Anyone like to comment as to whether it might be
> > usable for a limited IH operation?
> >
> > Thank you,
> >
> > Matthew Fleming, MD
> > Fleming Dermatopathology
> > Milwaukee, WI
> >
>
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