[Histonet] Bouin postfixation

[SANCHEZ QUINTEIRO PABLO]

Thanks for your input and for your curiosity Carl,

The antibody is raised against G protein subunit alpha i2. Made by Santa Cruz Biotechnologies SC-7276.

I have employed it for several years and from my experience with my protocols it works much better in Bouin's than in formalin.

The embedding is in paraffin.

Pablo

________________________________
De: Hobbs, Carl via Histonet <histonet at lists.utsouthwestern.edu>
Enviado: lunes, 4 de junio de 2018 21:21:32
Para: histonet at lists.utsouthwestern.edu
Asunto: Re: [Histonet] Bouin postfixation


Hi Pablo

What is the name, source and catalogue number  of your antibody?

( maybe you do not need to fix in Bouin's fixing fluid....do you know, as a fact, that Bouin's fix is better?)


Imho, once you have fixed in Formalin ( depending on the time in fixing fluid), you cannot successfully "refix" in another fixative.


Also: "embedding": do you mean in OCT or Pwax?


Curious-illy

Carl


Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson CARD
Guys Campus, London Bridge
Kings College London
London
SE1 1UL

020 7848 6813


________________________________
From: histonet-request at lists.utsouthwestern.edu <histonet-request at lists.utsouthwestern.edu>
Sent: 04 June 2018 18:00
To: histonet at lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 175, Issue 3

Send Histonet mailing list submissions to
        histonet at lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
        https://emea01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=01%7C01%7Ccarl.hobbs%40kcl.ac.uk%7C3fc139402a6e4a115ffa08d5ca3f90d2%7C8370cf1416f34c16b83c724071654356%7C0&sdata=lkgAB3JHrCmogfTpMGuXOjWVnYxZqGLoE7DKkcRxsD0%3D&reserved=0
or, via email, send a message with subject or body 'help' to
        histonet-request at lists.utsouthwestern.edu

You can reach the person managing the list at
        histonet-owner at lists.utsouthwestern.edu

When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."


Today's Topics:

   1. Bouin postfixation (SANCHEZ QUINTEIRO PABLO)


----------------------------------------------------------------------

Message: 1
Date: Sun, 3 Jun 2018 18:00:43 +0000
From: SANCHEZ QUINTEIRO PABLO <pablo.sanchez at usc.es>
To: "histonet at lists.utsouthwestern.edu"
        <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Bouin postfixation
Message-ID:
        <AM0PR04MB39856CD03F81CA6CD26FA42291600 at AM0PR04MB3985.eurprd04.prod.outlook.com>

Content-Type: text/plain; charset="iso-8859-1"


Dear histonetters,

I have to do immunohistochemistry with an antibody that works better in Bouin fixed samples. But I am afraid that my samples -just unprocessed- are in formalin.

Do you think that it could be help to transfer them to Bouin 24 hours and then 70 ethanol and embedding?

Thanks in advance

Pablo Sanchez-Quinteiro
Lugo. Spain


------------------------------

Subject: Digest Footer

_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
https://emea01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=01%7C01%7Ccarl.hobbs%40kcl.ac.uk%7C3fc139402a6e4a115ffa08d5ca3f90d2%7C8370cf1416f34c16b83c724071654356%7C0&sdata=lkgAB3JHrCmogfTpMGuXOjWVnYxZqGLoE7DKkcRxsD0%3D&reserved=0

------------------------------

End of Histonet Digest, Vol 175, Issue 3
****************************************
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet