[Histonet] Bouin postfixation

[Hobbs, Carl]

Hi Pablo

What is the name, source and catalogue number  of your antibody?

( maybe you do not need to fix in Bouin's fixing fluid....do you know, as a fact, that Bouin's fix is better?)


Imho, once you have fixed in Formalin ( depending on the time in fixing fluid), you cannot successfully "refix" in another fixative.


Also: "embedding": do you mean in OCT or Pwax?


Curious-illy

Carl


Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson CARD
Guys Campus, London Bridge
Kings College London
London
SE1 1UL

020 7848 6813


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Today's Topics:

   1. Bouin postfixation (SANCHEZ QUINTEIRO PABLO)


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Message: 1
Date: Sun, 3 Jun 2018 18:00:43 +0000
From: SANCHEZ QUINTEIRO PABLO <pablo.sanchez at usc.es>
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Subject: [Histonet] Bouin postfixation
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Dear histonetters,

I have to do immunohistochemistry with an antibody that works better in Bouin fixed samples. But I am afraid that my samples -just unprocessed- are in formalin.

Do you think that it could be help to transfer them to Bouin 24 hours and then 70 ethanol and embedding?

Thanks in advance

Pablo Sanchez-Quinteiro
Lugo. Spain


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