[Histonet] osmium tetroxide

Logan, Shannon Shannon.Logan at bellin.org
Wed Aug 29 09:49:16 CDT 2018

Osmium Tetroxide is highly toxic. Storage is an issue and working with it, you must be under a hood with eye protection. I think most (if not all) labs have banned this substance? If you do not “have” to work with this material, I would recommend avoiding it. Just my opinion, thanks…

Shannon H. Logan  B.S., HTL (ASCP)
Pathology Department

Bellin Health Memorial Hospital
744 South Webster Avenue
Green Bay, WI 54305-3400
920-433-3653  X3727

From: Blanca Lopez via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Wednesday, August 29, 2018 8:11 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] osmium tetroxide

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I am looking a protocol to stain osmium tetroxide....Is anybody can share their thoughts...on a frozen section.

I attached an idea of how this person wants me to follow his protocol with this staining but I kind of lost...

my PI request:
I'll be perfusing the mice with saline solution 0.9% followed by 10% formalin
Cryoprotect the tissue with 30% sucrose
Cut 30 micron sections in cryostat and mount on slides

I would handle you the slides, since the tissue contains Fluorescent proteins, must be protected from light

Once the staining with osmium is finished, you can give me the slides back to check the staining and the fluorescence before it is dehydrated and coverslip.

thanks for your help

Blanca Lopez HT (ASCP)
UT Southwestern Medical Center
Harold C. Simmons Comprehensive Cancer Center
Histology Lab
blanca.lopez at utsouthwestern.edu<mailto:blanca.lopez at utsouthwestern.edu<mailto:blanca.lopez at utsouthwestern.edu%3cmailto:blanca.lopez at utsouthwestern.edu>>


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