[Histonet] osmium tetroxide

[Hawkins, Hal K.]

I would suspect osmium would destroy the fluorescent proteins the PI wants to detect, while staining fat droplets nicely.  You can always ask your friendly EM lab to do the staining to find out, they have to use it and are familiar with the proper ways to deal with it.

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From: Logan, Shannon via Histonet [histonet at lists.utsouthwestern.edu]
Sent: Wednesday, August 29, 2018 9:49 AM
To: Blanca Lopez
Cc: histonet (histonet at lists.utsouthwestern.edu)
Subject: Re: [Histonet] osmium tetroxide

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Osmium Tetroxide is highly toxic. Storage is an issue and working with it, you must be under a hood with eye protection. I think most (if not all) labs have banned this substance? If you do not “have” to work with this material, I would recommend avoiding it. Just my opinion, thanks…


Shannon H. Logan  B.S., HTL (ASCP)
Pathology Department

Bellin Health Memorial Hospital
744 South Webster Avenue
Green Bay, WI 54305-3400
920-433-3653  X3727




From: Blanca Lopez via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Wednesday, August 29, 2018 8:11 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] osmium tetroxide


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Histotechs:
I am looking a protocol to stain osmium tetroxide....Is anybody can share their thoughts...on a frozen section.


I attached an idea of how this person wants me to follow his protocol with this staining but I kind of lost...


my PI request:
I'll be perfusing the mice with saline solution 0.9% followed by 10% formalin
Cryoprotect the tissue with 30% sucrose
Cut 30 micron sections in cryostat and mount on slides


I would handle you the slides, since the tissue contains Fluorescent proteins, must be protected from light


Once the staining with osmium is finished, you can give me the slides back to check the staining and the fluorescence before it is dehydrated and coverslip.


thanks for your help


Blanca Lopez HT (ASCP)
Histotechnologist
UT Southwestern Medical Center
Harold C. Simmons Comprehensive Cancer Center
Histology Lab
K1-210
214-648-7598
blanca.lopez at utsouthwestern.edu<mailto:blanca.lopez at utsouthwestern.edu<mailto:blanca.lopez at utsouthwestern.edu%3cmailto:blanca.lopez at utsouthwestern.edu>>



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