[Histonet] Alcian Blue digested w Hyaluronidase - any alternatives?

[Hobbs, Carl]

You are right!

Thank you for correcting me.

Surely sialomucins will be positive for PAS and H.acid will be negative?


Curious-illy


Carl


Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson CARD
Guys Campus, London Bridge
Kings College London
London
SE1 1UL

020 7848 6813
________________________________
From: Mac Donald, Jennifer <jmacdonald at mtsac.edu>
Sent: 23 August 2018 19:52:52
To: Hobbs, Carl
Cc: histonet at lists.utsouthwestern.edu
Subject: RE: [Histonet] Alcian Blue digested w Hyaluronidase - any alternatives?

The hyaluronidase is digesting out hyaluronic acid, a component found in connective tissue mucins.  This reaction is used to differentiate connective tissue mucins from epithelial mucins.  Hyaluronic acid and epithelial sialomucins will give the same staining results, both positive for alcian blue pH 2.5 and negative for pH 1.0.

-----Original Message-----
From: Hobbs, Carl via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Thursday, August 23, 2018 11:32 AM
To: histonet <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Alcian Blue digested w Hyaluronidase - any alternatives?

Perhaps Alcian blue at pH3 ( all acidic mucins) on one slide/section  and Ab pH1 ( highly sulphated mucins only) on a sequential slide?
Sure, instead  use Alcian blue CEC method?
I will be interested to read other responses to your request.

Mucin-illy

carl




Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson CARD
Guys Campus, London Bridge
Kings College London
London
SE1 1UL

020 7848 6813
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
https://emea01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=01%7C01%7Ccarl.hobbs%40kcl.ac.uk%7Cc3bfd2048e1b4508a26b08d60929a33c%7C8370cf1416f34c16b83c724071654356%7C0&sdata=CTVwv5AklHrbGKIoml5W4NVoYJ7Skdfro9F%2FXE0uuWM%3D&reserved=0