[Histonet] Alcian Blue digested w Hyaluronidase - any alternatives?
Mac Donald, Jennifer
jmacdonald at mtsac.edu
Thu Aug 23 13:52:52 CDT 2018
The hyaluronidase is digesting out hyaluronic acid, a component found in connective tissue mucins. This reaction is used to differentiate connective tissue mucins from epithelial mucins. Hyaluronic acid and epithelial sialomucins will give the same staining results, both positive for alcian blue pH 2.5 and negative for pH 1.0.
From: Hobbs, Carl via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Thursday, August 23, 2018 11:32 AM
To: histonet <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Alcian Blue digested w Hyaluronidase - any alternatives?
Perhaps Alcian blue at pH3 ( all acidic mucins) on one slide/section and Ab pH1 ( highly sulphated mucins only) on a sequential slide?
Sure, instead use Alcian blue CEC method?
I will be interested to read other responses to your request.
Carl Hobbs FIBMS
Histology and Imaging Manager
Guys Campus, London Bridge
Kings College London
020 7848 6813
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