[Histonet] Alcian Blue digested w Hyaluronidase - any alternatives?

[Mac Donald, Jennifer]

The hyaluronidase is digesting out hyaluronic acid, a component found in connective tissue mucins.  This reaction is used to differentiate connective tissue mucins from epithelial mucins.  Hyaluronic acid and epithelial sialomucins will give the same staining results, both positive for alcian blue pH 2.5 and negative for pH 1.0.

-----Original Message-----
From: Hobbs, Carl via Histonet <histonet at lists.utsouthwestern.edu> 
Sent: Thursday, August 23, 2018 11:32 AM
To: histonet <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Alcian Blue digested w Hyaluronidase - any alternatives?

Perhaps Alcian blue at pH3 ( all acidic mucins) on one slide/section  and Ab pH1 ( highly sulphated mucins only) on a sequential slide?
Sure, instead  use Alcian blue CEC method?
I will be interested to read other responses to your request.

Mucin-illy

carl


  
 
Carl Hobbs FIBMS 
Histology and Imaging Manager 
Wolfson CARD 
Guys Campus, London Bridge  
Kings College London 
London 
SE1 1UL 
  
020 7848 6813    
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