[Histonet] Alcian Blue digested w Hyaluronidase - any alternatives?

[Hobbs, Carl]

Vielen dank, Michael

Very kind of you to send this to me.

Alcian blue is indeed an interesting dye

I thought that it was no longer manufactured?

I am wrong, obviously.

I have used the CEC method many years ago when analysing articular cartilages and it is good to see that it is still a valid method to differentiate the various acidic mucins


Alles Gute


carl


Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson CARD
Guys Campus, London Bridge
Kings College London
London
SE1 1UL

020 7848 6813
________________________________
From: Dr. Michael Gudo (Morphisto GmbH) <michael.gudo at morphisto.de>
Sent: 24 August 2018 11:22:55
To: Hobbs, Carl
Cc: histonet
Subject: Re: [Histonet] Alcian Blue digested w Hyaluronidase - any alternatives?

Hello Carl,

Alcianblue is q quite interesting stain! Maybe this selection of a presentation helps you to find a suitable method. I attach you a PDF-document to this email. The slides are in german, but I think you will understand them anyway.

Kind regards
Michael


Am 23.08.2018 um 20:32 schrieb Hobbs, Carl via Histonet <histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu>>:

Perhaps Alcian blue at pH3 ( all acidic mucins) on one slide/section  and Ab pH1 ( highly sulphated mucins only) on a sequential slide?
Sure, instead  use Alcian blue CEC method?
I will be interested to read other responses to your request.

Mucin-illy

carl




Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson CARD
Guys Campus, London Bridge
Kings College London
London
SE1 1UL

020 7848 6813
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