[Histonet] Iron Stain

Caroline Miller mills at 3scan.com
Wed Aug 8 14:52:48 CDT 2018

HI Tasha,

The nuclear fast red will stain the nuclei red, the biebrich scarlet is a
connective tissue stain and may cover up the (sometimes sparse) prussian
blue localization of iron in the tissues. I, personally, would not consider
these reagents swappable.

In my experience nuclear fast red does last a long time, but I am also not
in a lab where expiration dates matter.


On Wed, Aug 8, 2018 at 4:52 AM Campbell, Tasha M. via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Is nuclear fast red the only counter stain for Prussian blue stain?  I
> have a Masson's trichrome kit and was wondering if the Bierbech Scarlet
> could be a counterstain.  I won't be doing the iron stain very often at all
> so I am trying to keep dry reagents on hand to make up as needed so they do
> not expire so quickly.  Thanks!
> Tasha Campbell, B.S.,HTL(ASCP)
> Frederick Gastroenterology Associates
> 310 W. 9th St.
> Frederick, MD 21701
> 301-695-6800 ext. 144
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Caroline Miller (mills)
Director of Biology

415 2187297

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