[Histonet] Iron Stain

[Caroline Miller]

Oops, yes, I am mixing up neutral red - and the recipe from Bryan is what I
use - it last forever.

I have had luck with nuclear fast red from vector - comes made up. That
lasts a little longer than lab made.

mills
ᐧ

On Wed, Aug 8, 2018 at 12:52 PM Caroline Miller <mills at 3scan.com> wrote:

> HI Tasha,
>
> The nuclear fast red will stain the nuclei red, the biebrich scarlet is a
> connective tissue stain and may cover up the (sometimes sparse) prussian
> blue localization of iron in the tissues. I, personally, would not consider
> these reagents swappable.
>
> In my experience nuclear fast red does last a long time, but I am also not
> in a lab where expiration dates matter.
>
> mills
> ᐧ
>
> On Wed, Aug 8, 2018 at 4:52 AM Campbell, Tasha M. via Histonet <
> histonet at lists.utsouthwestern.edu> wrote:
>
>> Is nuclear fast red the only counter stain for Prussian blue stain?  I
>> have a Masson's trichrome kit and was wondering if the Bierbech Scarlet
>> could be a counterstain.  I won't be doing the iron stain very often at all
>> so I am trying to keep dry reagents on hand to make up as needed so they do
>> not expire so quickly.  Thanks!
>>
>>
>>
>>
>> Tasha Campbell, B.S.,HTL(ASCP)
>> Frederick Gastroenterology Associates
>> 310 W. 9th St.
>> Frederick, MD 21701
>> 301-695-6800 ext. 144
>>
>> _______________________________________________
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>>
>
>
> --
> Caroline Miller (mills)
> Director of Biology
>
> www.3Scan.com
> 415 2187297
>
>
>

-- 
Caroline Miller (mills)
Director of Biology

www.3Scan.com
415 2187297