[Histonet] FW: cutting a 5um frozen section from a 50um section of brain

[Morken, Timothy]

Haley, 

Maybe ask Stephen directly. He is on histonet, and has a website:  https://www.pathologyinnovations.com/


Tim Morken
Pathology Site Manager, Parnassus 
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center

-----Original Message-----
From: Haley Huggins via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Friday, October 06, 2017 10:31 AM
To: Dessasau III, Evan
Cc: histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] FW: cutting a 5um frozen section from a 50um section of brain

I would also be interested in knowing this tip if anyone knows it. I will also have to check out that book. We only do cryostat sections at our lab.

*Haley Huggins, HT (ASCP)cm*
*Technical Lab Supervisor*
*1050 Las Tablas Rd, Suite 14*
*Templeton, CA 93465*
*Office: 877-230-1518*

On Fri, Oct 6, 2017 at 9:09 AM, Dessasau III, Evan via Histonet < histonet at lists.utsouthwestern.edu> wrote:

> Hi Histonet , I have been trying to cut a FLAT 4 to 10 um frozen 
> section from 50um sections of brain.  Every time I think I have the 
> tissue flat the sections are never in the same plane.  I found a 
> wonderful book in pdf format(A Practical Guide to Frozen Section 
> Techniques, Stephen R. Peters) online with lots of wonderful tips but 
> I'm having no luck implementing the tips.  Has anyone ever tried this?  Any help is GREATLY appreciated.
> Thank you,
> E-van
>
> E-van D. Dessasau, III, HTL(ASCP)cm
> Supervisor, Histology Division of Pathology Emory University Yerkes 
> NPRC Main Center Rm. 2122
> 954 Gatewood Rd.
> Atlanta, GA. 30329
> (404)727-7744 lab
> (404) 727-7902 office
>
>
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