[Histonet] FW: cutting a 5um frozen section from a 50um section of brain

Haley Huggins hhuggins at novalabs.co
Fri Oct 6 12:30:53 CDT 2017

I would also be interested in knowing this tip if anyone knows it. I will
also have to check out that book. We only do cryostat sections at our lab.

*Haley Huggins, HT (ASCP)cm*
*Technical Lab Supervisor*
*1050 Las Tablas Rd, Suite 14*
*Templeton, CA 93465*
*Office: 877-230-1518*

On Fri, Oct 6, 2017 at 9:09 AM, Dessasau III, Evan via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Hi Histonet , I have been trying to cut a FLAT 4 to 10 um frozen section
> from 50um sections of brain.  Every time I think I have the tissue flat the
> sections are never in the same plane.  I found a wonderful book in pdf
> format(A Practical Guide to Frozen Section Techniques, Stephen R. Peters)
> online with lots of wonderful tips but I'm having no luck implementing the
> tips.  Has anyone ever tried this?  Any help is GREATLY appreciated.
> Thank you,
> E-van
> E-van D. Dessasau, III, HTL(ASCP)cm
> Supervisor, Histology Division of Pathology
> Emory University
> Yerkes NPRC
> Main Center Rm. 2122
> 954 Gatewood Rd.
> Atlanta, GA. 30329
> (404)727-7744 lab
> (404) 727-7902 office
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