[Histonet] EDTA decalcification tissue issues

[Cartun, Richard]

We switched from acid decal to EDTA recently; primarily for molecular testing.  We are starting to see some excellent results.  At first, we were not decaling long enough (EDTA decalcification is a very slow process).  We also use the Leica Bond Max and we have not had any problems with tissue loss.  We are only using EDTA for small biopsies.  Please not that these specimens must be fixed adequately before the decal process is started.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax

-----Original Message-----
From: Pairan, Kelly via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Friday, November 10, 2017 6:39 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] EDTA decalcification tissue issues

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Good Morning,

Recently, our lab has been working on validating an EDTA method of decalcification.  When we ran the IHC's on the decalcified bone block, the majority of the tissue lifted off the slide.  We use the Leica Bonds for our IHC staining.  Does anyone else have a hard time getting EDTA decalcified tissue to stay on positively charged slides during IHC runs?  Do you have a trick you use?  We really did not have this big of a problem when we were decalcifying with Formical or Nitrical (big bones only).


Thanks for your help,

?Kelly Pairan, HT(ASCP), QIHC(ASCP)
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