[Histonet] EDTA decalcification tissue issues

Dorothy Hu abtdhu at gmail.com
Mon Nov 13 08:52:21 CST 2017


We didn't compare EDTA with formic acid. But heard that formic acid also
can do many IHC and ISH, if not all the antibodies and all probes. I always
have problem of bone falling off, no matter what kind of slides. Thinking
both PFA and EDTA affect on this issue. So trying frozen tape unfixed,
undecalcified bone now. If anyone has research paper regarding this, please
share.
Thanks.

Dorothy Hu


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