[Histonet] Metal embedding molds-large

[Bryan Llewellyn]

Sorry!

That should be TSP - trisodium phosphate - not TCP, which might make it 
worse.

Bryan


Bryan Llewellyn wrote:
> This used to be a common problem years ago. It is due to crud buildup on
> the metal. Boil them with TCP for half an hour, then thoroughly wash
> them in cold water. Coat them with a VERY light smear of glycerol before
> you use them, preferably each time. That should help.
>
> Bryan Llewellyn.
>
> Diane Satterfield via Histonet wrote:
>> We are using large metal molds to embed mouse brains.  We are having a
>> hard time getting to block out of the molds, the paraffin blocks are
>> sticking.  Sometimes they are coming out cracked.  Sometimes the
>> cassette comes off the paraffin block.  Any idea why this is
>> happening? Any advice on how to fix this problem?
>>
>>
>> Diane L. Satterfield, BS
>> Manager Brain Tumor BioRepository
>> Research Program Leader
>> Duke University Medical Center
>> Brain Tumor Center Biorepository and Database
>>
>> diane.satterfield at duke.edu<mailto:diane.satterfield at duke.edu>
>> office  919-684-4642
>> pager  919-970-7328
>> fax  919-684-4975
>>
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>