[Histonet] Metal embedding molds-large

[Caroline Miller]

hey, we do all our embedding in those molds, and here is what I suggest:

1 - Make sure to have enough wax in the back of the molds, all the way
until it is on the lip of the cassette - you may need to refill a  bunch of
times because the wax drains out (why regular sakura cassettes do not fit
in these molds, also made by sakura I really don't know). But you need to
wait until it hardens enough, but not too much to leave a transition
between the two fill waxes. This will prevent the cassette from coming away
when you pop it out
2 - Make sure the block is nice and cold, wait 10 minutes longer than you
think you have to
3 - Use a spatula or other strong item to place under the label side of the
cassette and pop out of the mold, if you get any resistance then WAIT some
more! (again them being cold is really important)

good luck, happy to answer any clarifying questions!

mills

On Wed, Nov 8, 2017 at 12:30 PM, Bryan Llewellyn via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Sorry!
>
> That should be TSP - trisodium phosphate - not TCP, which might make it
> worse.
>
> Bryan
>
>
>
> Bryan Llewellyn wrote:
>
>> This used to be a common problem years ago. It is due to crud buildup on
>> the metal. Boil them with TCP for half an hour, then thoroughly wash
>> them in cold water. Coat them with a VERY light smear of glycerol before
>> you use them, preferably each time. That should help.
>>
>> Bryan Llewellyn.
>>
>> Diane Satterfield via Histonet wrote:
>>
>>> We are using large metal molds to embed mouse brains.  We are having a
>>> hard time getting to block out of the molds, the paraffin blocks are
>>> sticking.  Sometimes they are coming out cracked.  Sometimes the
>>> cassette comes off the paraffin block.  Any idea why this is
>>> happening? Any advice on how to fix this problem?
>>>
>>>
>>> Diane L. Satterfield, BS
>>> Manager Brain Tumor BioRepository
>>> Research Program Leader
>>> Duke University Medical Center
>>> Brain Tumor Center Biorepository and Database
>>>
>>> diane.satterfield at duke.edu<mailto:diane.satterfield at duke.edu>
>>> office  919-684-4642
>>> pager  919-970-7328
>>> fax  919-684-4975
>>>
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>
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-- 
Caroline Miller (mills)
Director of Histology
3Scan.com
415 2187297