[Histonet] Metal embedding molds-large

[Bryan Llewellyn]

This used to be a common problem years ago. It is due to crud buildup on 
the metal. Boil them with TCP for half an hour, then thoroughly wash 
them in cold water. Coat them with a VERY light smear of glycerol before 
you use them, preferably each time. That should help.

Bryan Llewellyn.

Diane Satterfield via Histonet wrote:
> We are using large metal molds to embed mouse brains.  We are having a hard time getting to block out of the molds, the paraffin blocks are sticking.  Sometimes they are coming out cracked.  Sometimes the cassette comes off the paraffin block.  Any idea why this is happening? Any advice on how to fix this problem?
>
>
> Diane L. Satterfield, BS
> Manager Brain Tumor BioRepository
> Research Program Leader
> Duke University Medical Center
> Brain Tumor Center Biorepository and Database
>
> diane.satterfield at duke.edu<mailto:diane.satterfield at duke.edu>
> office  919-684-4642
> pager  919-970-7328
> fax  919-684-4975
>
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