[Histonet] Histonet Digest, Vol 162, Issue 26 butterflies

Steve McClain SteveM at mcclainlab.com
Wed May 31 05:08:50 CDT 2017

I have no experiences w butterflies, but the chitin in ticks, carpenter ants and spiders does not cut well.  
For insects and arthropods we post-fix in Carnoy's for 60 minutes
Then soften with 10% KOH RT for 10-20 minutes 
then wash for 30-60minutes in water. Long processing cycle. 

Steve A. McClain, MD

On May 30, 2017, at 13:26, "histonet-request at lists.utsouthwestern.edu" <histonet-request at lists.utsouthwestern.edu> wrote:

>> Does anyone have any experience processing and paraffin embedding
>> butterflies?
>> Rhonda Gregoire, MLT
>> Supervisor, Clinical Pathology
>> Veterinary Diagnostic Services
>> Manitoba Agriculture
>> 545 University Crescent, Winnipeg, MB, R3T 5S6
>> Rhonda.Gregoire at gov.mb.ca<mailto:Rhonda.Gregoire at gov.mb.ca>
>> T : 204-945-7641  F : 204-945-7646

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