[Histonet] A Question About Paraffin Times

[cynthia haynes]

Please don't leave the tissue in the hot wax.They will harden and be brittle. Cynthia James H.T 

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  On Tue, May 16, 2017 at 10:50 AM, P Sicurello via Histonet<histonet at lists.utsouthwestern.edu> wrote:   Good Morning Listers,

I am asking the collective wisdom of the Histonet this question:


Is it better to remove baskets from the processor on Saturday morning and:

A.  Let the cassettes freeze, then melt them down and embed Monday morning?
OR
B.  Leave the cassettes in molten paraffin and embed Monday morning?


I am of the opinion that leaving the samples (not fatty, like breast cores)
in molten paraffin (62 degrees C)  is bad practice, and causes them to get
"crunchy", among other things.

What do you think?

Thank in advance.

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872 <#>



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