[Histonet] A Question About Paraffin Times

Logan, Shannon Shannon.Logan at bellin.org
Tue May 16 13:13:37 CDT 2017

Hello Paula,
So why must the cassettes be removed on Saturday morning if you aren't embedding until Monday morning?
Don't you have a "delay start" feature on your processor? Neither option A or B seem like a good thing for the tissue!
 We time our processor to finish at 5 AM Monday when the first Histotech arrives for embedding.
The cassettes remain in formalin until the processor starts up on Sunday evening.


Shannon H. Logan  B.S., HTL (ASCP)
Pathology Department

Bellin Health Memorial Hospital
744 South Webster Avenue
Green Bay, WI 54305-3400
920-433-3653  X3727

From: P Sicurello via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Tuesday, May 16, 2017 10:34 AM
To: HistoNet
Subject: [Histonet] A Question About Paraffin Times

Good Morning Listers,

I am asking the collective wisdom of the Histonet this question:

Is it better to remove baskets from the processor on Saturday morning and:

A. Let the cassettes freeze, then melt them down and embed Monday morning?
B. Leave the cassettes in molten paraffin and embed Monday morning?

I am of the opinion that leaving the samples (not fatty, like breast cores)
in molten paraffin (62 degrees C) is bad practice, and causes them to get
"crunchy", among other things.

What do you think?

Thank in advance.


Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872 <#>

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