[Histonet] A Question About Paraffin Times

Rene J Buesa rjbuesa at yahoo.com
Tue May 16 10:57:45 CDT 2017

I consider taking out → freezing →  melting → casting blocks is worse than leaving the tissues in molten paraffin.It seems that your Monday tissue processing ends on Saturday. I suggest to process your tissues with a "delay" (most tissue processers have this feature) and leave  them more time in formalin and make coincide their ending time with your Monday start time.René 

    On Tuesday, May 16, 2017 11:51 AM, P Sicurello via Histonet <histonet at lists.utsouthwestern.edu> wrote:

 Good Morning Listers,

I am asking the collective wisdom of the Histonet this question:

Is it better to remove baskets from the processor on Saturday morning and:

A.  Let the cassettes freeze, then melt them down and embed Monday morning?
B.  Leave the cassettes in molten paraffin and embed Monday morning?

I am of the opinion that leaving the samples (not fatty, like breast cores)
in molten paraffin (62 degrees C)  is bad practice, and causes them to get
"crunchy", among other things.

What do you think?

Thank in advance.


Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872 <#>

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