[Histonet] Update on Trimming soft brain

Julio Benavides j.benavides at eae.csic.es
Fri Mar 31 10:39:14 CDT 2017

Hi there,

just to update you on the issue. Thanks to the kind and useful 
suggestions from histonetes, I found the solution to my problem.

Fixation for two days in 10% formalin in absolute ethanol worked 
perfectly well. Brains were well fixed, we could trimmed them, and the 
fixation in alcoholic formalin did not interfere with the IHCs, at least 
with the one we are using (CD3, bAPP and GFAP).

Again, thank you so much for your help!



El 06/03/2017 a las 23:18, Julio Benavides via Histonet escribió:
> Hi there,
> I´m trying to trim ovine foetal brain (90 days of gestation). The 
> brain is
> too soft and there is no way to get a proper slice. It has been in 
> buffered
> 10% formalin for three weeks but still too soft. When you take the brain
> out of the pot and put into the trimming table, it just kind of spill 
> over
> the place. We have tried to embed the brain in 7.5% gelatin, which 
> helps to
> keep the shape of the brain before trimming, but as soon you cut the 
> slice,
> the centre of the tissue just melt away. I was wondering if there is any
> post fixative solution we can try. May be Bouin´s?
> Any suggestion would be greatly appreciated!
> Cheers
> Julio
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