[Histonet] need help staining 120um human whole brain sections!

[Rene J Buesa]

You have a special project → special tasks so your approach has to be equally special.Large brain sections are usually stained while floating but for IH with different and successive steps requiring very expensive reagents, floating sections is not well suited.You should affix the sections to large slides, and I imagine will not be brain whole sections but limited to some areas.In that case for IHC you can stain several sections in a humid chamber, manually. I do not imagine an automatic system for this task.It will be a costly and slow process indeed.René 

    On Sunday, March 19, 2017 8:27 PM, Maria Mejia via Histonet <histonet at lists.utsouthwestern.edu> wrote:
 

 
Or lab is currently processing a human whole brain.  In about a month or two, the whole brain, which will be encased 
in celloidin & serial sections will be cut at 120um each.  Now, we’ve bought an old Tetrander cast iron microtome. If 
you haven’t seen one of these microtomes, I can tell you it’s BIG!

Now, we’ll have to stain quite a number of these sections for IHC.  In fact too many to handle manually. If possible, need
to find a way to at least stain the majority of these sections in a semi-automatic system e.g. washes, quenching & blocking. 

Does any one think it’s possible to convert a LABGO processor (made in India & can hold 2 liter glass beakers) or a 
Sakura-Tissue-Tek 4640 (also holds glass beakers - both are old style circular processors using glass beakers for staining 
these sections? Does anyone have an alternative system? I could sure use some input or ideas anyone welcome
and most appreciated.

Maria Mejia
Lead Histologist
UCSF
Mission Bay
San Francisco, CA




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