[Histonet] Metabisulfite rinse for PAS reaction (potassium vs sodium)

[Angela Lamberth]

Thank you Philip. I'm writing a procedure and was driving myself bananas.

On Mon, Mar 6, 2017 at 11:01 AM, Manfre, Philip <philip_manfre at merck.com>
wrote:

> I almost always have used SODIUM metabisulfite for any version of PAS.  I
> am also familiar with procedures that go straight to tap water for the
> pot-Schiff's rinsing, though that was from a job in my past.  I seem to
> remember that working fine.
>
> Phil.
>
> Philip Manfre, HT (ASCP)
> Associate Principal Scientist
> Merck Research Laboratories
> WP81-406
> 770 Sumneytown Pike
> West Point, PA 19486
>
> 215-652-9750
> 215-993-0383 (fax)
> philip_manfre at merck.com
>
>
>
> -----Original Message-----
> From: Angela Lamberth via Histonet [mailto:histonet at lists.
> utsouthwestern.edu]
> Sent: Monday, March 06, 2017 12:58 PM
> To: histonet at lists.utsouthwestern.edu
> Subject: [Histonet] Metabisulfite rinse for PAS reaction (potassium vs
> sodium)
>
> In Carson’s 3rd edition, the reducing rinse following Schiff for PAS is
> 0.55% potassium metabisulfite (pg 137-138). For PAS/AB the reducing rinse
> is given as sodium metabisulfite (Carson pg 149).
>
>
>
> My other texts (Sheehan/Hrapchak as well as Vacca) list the reducing rinse
> as sodium metabisulfite which makes sense to me since the Schiff is made
> with sodium metabisulfite.
>
>
>
> Is this a printing error in the Carson book? I appreciate any light anybody
> can shed on this.
>
> --
> Angela Lamberth
> Histology Technician III
> Histology Core Lab
> La Jolla Institute for Allergy & Immunology
> 9420 Athena Circle
> La Jolla, CA 92037
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> Notice:  This e-mail message, together with any attachments, contains
> information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth,
> New Jersey, USA 07033), and/or its affiliates Direct contact information
> for affiliates is available at
> http://www.merck.com/contact/contacts.html) that may be confidential,
> proprietary copyrighted and/or legally privileged. It is intended solely
> for the use of the individual or entity named on this message. If you are
> not the intended recipient, and have received this message in error,
> please notify us immediately by reply e-mail and then delete it from
> your system.
>



-- 
Angela Lamberth
Histology Technician III
Histology Core Lab
La Jolla Institute for Allergy & Immunology
9420 Athena Circle
La Jolla, CA 92037