[Histonet] Metabisulfite rinse for PAS reaction (potassium vs sodium)
alamberth at lji.org
Mon Mar 6 13:12:47 CST 2017
Thank you Philip. I'm writing a procedure and was driving myself bananas.
On Mon, Mar 6, 2017 at 11:01 AM, Manfre, Philip <philip_manfre at merck.com>
> I almost always have used SODIUM metabisulfite for any version of PAS. I
> am also familiar with procedures that go straight to tap water for the
> pot-Schiff's rinsing, though that was from a job in my past. I seem to
> remember that working fine.
> Philip Manfre, HT (ASCP)
> Associate Principal Scientist
> Merck Research Laboratories
> 770 Sumneytown Pike
> West Point, PA 19486
> 215-993-0383 (fax)
> philip_manfre at merck.com
> -----Original Message-----
> From: Angela Lamberth via Histonet [mailto:histonet at lists.
> Sent: Monday, March 06, 2017 12:58 PM
> To: histonet at lists.utsouthwestern.edu
> Subject: [Histonet] Metabisulfite rinse for PAS reaction (potassium vs
> In Carson’s 3rd edition, the reducing rinse following Schiff for PAS is
> 0.55% potassium metabisulfite (pg 137-138). For PAS/AB the reducing rinse
> is given as sodium metabisulfite (Carson pg 149).
> My other texts (Sheehan/Hrapchak as well as Vacca) list the reducing rinse
> as sodium metabisulfite which makes sense to me since the Schiff is made
> with sodium metabisulfite.
> Is this a printing error in the Carson book? I appreciate any light anybody
> can shed on this.
> Angela Lamberth
> Histology Technician III
> Histology Core Lab
> La Jolla Institute for Allergy & Immunology
> 9420 Athena Circle
> La Jolla, CA 92037
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Histology Technician III
Histology Core Lab
La Jolla Institute for Allergy & Immunology
9420 Athena Circle
La Jolla, CA 92037
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