[Histonet] Metabisulfite rinse for PAS reaction (potassium vs sodium)

[Manfre, Philip]

I almost always have used SODIUM metabisulfite for any version of PAS.  I am also familiar with procedures that go straight to tap water for the pot-Schiff's rinsing, though that was from a job in my past.  I seem to remember that working fine.

Phil.

Philip Manfre, HT (ASCP)
Associate Principal Scientist
Merck Research Laboratories
WP81-406
770 Sumneytown Pike
West Point, PA 19486

215-652-9750
215-993-0383 (fax)
philip_manfre at merck.com



-----Original Message-----
From: Angela Lamberth via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Monday, March 06, 2017 12:58 PM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Metabisulfite rinse for PAS reaction (potassium vs sodium)

In Carson’s 3rd edition, the reducing rinse following Schiff for PAS is
0.55% potassium metabisulfite (pg 137-138). For PAS/AB the reducing rinse
is given as sodium metabisulfite (Carson pg 149).



My other texts (Sheehan/Hrapchak as well as Vacca) list the reducing rinse
as sodium metabisulfite which makes sense to me since the Schiff is made
with sodium metabisulfite.



Is this a printing error in the Carson book? I appreciate any light anybody
can shed on this.

-- 
Angela Lamberth
Histology Technician III
Histology Core Lab
La Jolla Institute for Allergy & Immunology
9420 Athena Circle
La Jolla, CA 92037
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