[Histonet] Dirty H&E after break

[Tony Henwood (SCHN)]

Hi Allan,

The flakes are alum precipitates.

I would suggest filtering the haematoxylin before use.

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Principal Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: Allan Wang via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Wednesday, 4 January 2017 4:45 PM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Dirty H&E after break

Hello all,

Does someone know what would cause dirty flakes of light purple that showed across the whole slide?

http://i.imgur.com/1nIXwAy.jpg

Reagents are relatively fresh but the H&E stainer hadn't been used for a week over the holiday break.
Is it surface film on the hematoxylin? I didn't notice anything in the container. We are using Mayer's, followed by differentiation with HCl acid alcohol, with 2-3 minute long wash steps in between.

Thanks,
Allan
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