[Histonet] Dirty H&E after break

[Allan Wang]

Yep, filtering the hematoxlyn fixed it. Thanks for the tip.

Is it worthwhile to filter the eosin, alcohols, and xylene substitutes as
well?

Allan


On Jan 4, 2017 1:04 AM, "Tony Henwood (SCHN)" <
tony.henwood at health.nsw.gov.au> wrote:

> Hi Allan,
>
> The flakes are alum precipitates.
>
> I would suggest filtering the haematoxylin before use.
>
> Regards
> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
> Principal Scientist, the Children's Hospital at Westmead
> Adjunct Fellow, School of Medicine, University of Western Sydney
> Tel: 612 9845 3306
> Fax: 612 9845 3318
> Pathology Department
> the children's hospital at westmead
> Cnr Hawkesbury Road and Hainsworth Street, Westmead
> Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
>
> -----Original Message-----
> From: Allan Wang via Histonet [mailto:histonet at lists.utsouthwestern.edu]
> Sent: Wednesday, 4 January 2017 4:45 PM
> To: histonet at lists.utsouthwestern.edu
> Subject: [Histonet] Dirty H&E after break
>
> Hello all,
>
> Does someone know what would cause dirty flakes of light purple that
> showed across the whole slide?
>
> http://i.imgur.com/1nIXwAy.jpg
>
> Reagents are relatively fresh but the H&E stainer hadn't been used for a
> week over the holiday break.
> Is it surface film on the hematoxylin? I didn't notice anything in the
> container. We are using Mayer's, followed by differentiation with HCl acid
> alcohol, with 2-3 minute long wash steps in between.
>
> Thanks,
> Allan
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