[Histonet] Dirty H&E after break

Allan Wang allanvv at gmail.com
Tue Jan 3 23:45:17 CST 2017


Hello all,

Does someone know what would cause dirty flakes of light purple that showed
across the whole slide?

http://i.imgur.com/1nIXwAy.jpg

Reagents are relatively fresh but the H&E stainer hadn't been used for a
week over the holiday break.
Is it surface film on the hematoxylin? I didn't notice anything in the
container. We are using Mayer's, followed by differentiation with HCl acid
alcohol, with 2-3 minute long wash steps in between.

Thanks,
Allan


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