[Histonet] Dirty H&E after break
Allan Wang
allanvv at gmail.com
Tue Jan 3 23:45:17 CST 2017
Hello all,
Does someone know what would cause dirty flakes of light purple that showed
across the whole slide?
http://i.imgur.com/1nIXwAy.jpg
Reagents are relatively fresh but the H&E stainer hadn't been used for a
week over the holiday break.
Is it surface film on the hematoxylin? I didn't notice anything in the
container. We are using Mayer's, followed by differentiation with HCl acid
alcohol, with 2-3 minute long wash steps in between.
Thanks,
Allan
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