[Histonet] Frog tissue

[koellingr at comcast.net]

Hello, 

For years and years in past, I did the very same thing for a middle school science teacher before, during and after son was there. Don't know what level this is at so unless all the kids are interested in histology, I've found no need to go too far with this (are they really going to learn about gut and lung and heart histology) based on the slides? 

I collected a fresh frog from teacher and fixed in 70% isopropyl from drug store. Worked great with just "normal histology processing" for level they were at. 

One really neat thing to look at is two sections of skin, embedded on edge. From a non-spotted area and a spotted skin area. The kids can really appreciate, at microscopic level and referring back to macro, the distribution of melanophores that cause the spots. I was really impressed when I first saw it. All the individual cells with pigment. 

Ray 

Raymond Koelling 
Lecturer, University of Washington School of Medicine, WWAMI, Spokane, WA 

----- Original Message -----

From: "John Shelley via Histonet" <histonet at lists.utsouthwestern.edu> 
To: histonet at lists.utsouthwestern.edu 
Sent: Tuesday, February 21, 2017 8:26:05 AM 
Subject: [Histonet] Frog tissue 

Good Morning Histonetters, 

My daughter's class dissected frogs and her teacher gave me a frog to dissect and make histological slides and blocks for her. I have taken a bunch of tissue and not too sure how well preserved the tissue may be but I thought I would give it a try. I am sure that someone out on the Histonet has encountered such tissue and would like some help with what a good processing schedule would be. I tried to cut everything between 3-4 mm but may have some a slight bit thicker. I have all internal organs like heart, lung, kidney, stomach, intestines, female reproductive system and some eggs. I have outer skin, an eye and both front and back hands and feet for a better term, along with tongue and upper jaw of mouth. I have decaled some bone. So as you can see I have an array of tissue types that may or may not require different schedules on the tissue processor and any help would be greatly appreciated. 

I thank you in advance! 

Kind Regards! 

John J Shelley 

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