[Histonet] hand staining immunos
Caroline Miller
mills at 3scan.com
Mon Feb 13 10:54:31 CST 2017
Agreed! Don't believe the 'single use only' on the coverplates - wash and
reuse them. I use tube racks to stand them up to dry, then put the back in
the original packaging. after a few years some of them might lose their
spring and you will need to replace some at that point.
yours,
mills
On Mon, Feb 13, 2017 at 7:42 AM, Paula Keene Pierce <
paula at excaliburpathology.com> wrote:
> https://www.fishersci.com/shop/products/7219950/7219950#?keyword=sequenza
>
> These are the cover plates.
>
> I have no financial interest in Thermo Fisher, but have used the system
> for years.
>
>
>
>
> Paula Keene Pierce, BS, HTL(ASCP)HT
> President
> Excalibur Pathology, Inc.
> 5830 N Blue Lake Drive
> Norman, OK 73069
> PH 405-759-3953 <(405)%20759-3953>
> FAX 405-759-7513 <(405)%20759-7513>
> www.excaliburpathology.com
>
>
> ------------------------------
> *From:* Caroline Miller via Histonet <histonet at lists.utsouthwestern.edu>
> *To:* Ranna Mehta <ranna0726 at gmail.com>
> *Cc:* "Histonet at Lists. Edu" <histonet at lists.utsouthwestern.edu>
> *Sent:* Monday, February 13, 2017 9:06 AM
> *Subject:* Re: [Histonet] hand staining immunos
>
> This is a great system for semi-automated immuno. I find the results a
> little cleaner than performing the technique straight on the slide. No need
> to buy the whole set up (it is just a glorified timer)- just the racks and
> clips are enough!
>
> https://www.fishersci.com/shop/products/thermo-
> scientific-shandon-sequenza-immunostaining-center-2/p-4010433
>
> mills
>
> On Thu, Feb 9, 2017 at 11:32 AM, Ranna Mehta via Histonet <
> histonet at lists.utsouthwestern.edu> wrote:
>
> > Hi Jennifer,
> >
> > How many slides are you planning to stain in routine? Once you will
> > understand the basic, it will be a very interesting field. If you like, I
> > can help you to walk through process with step by step. send me a message
> > through my email.All the best and thanks.
> >
> > Regards
> > Ranna
> >
> > On Thu, Feb 9, 2017 at 12:00 PM, <histonet-request at lists.
> > utsouthwestern.edu>
> > wrote:
> >
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> > > Today's Topics:
> > >
> > > 1. Looking for a Histology Workshop (Jessica Riggleman)
> > > 2. Re: Looking for a Histology Workshop (Jessica Riggleman)
> > > 3. hand staining immunos (Jennifer)
> > > 4. Re: hand staining immunos (Rene J Buesa)
> > > 5. Re: hand staining immunos (Walter Benton)
> > >
> > >
> > > ----------------------------------------------------------------------
> > >
> > > Message: 1
> > > Date: Wed, 8 Feb 2017 18:07:24 +0000
> > > From: Jessica Riggleman <jriggleman at globusmedical.com>
> > > To: "histonet at lists.utsouthwestern.edu"
> > > <histonet at lists.utsouthwestern.edu>
> > > Subject: [Histonet] Looking for a Histology Workshop
> > > Message-ID:
> > > <D0AEE53F1F084340888BEB10C81DE9B295C77FAC at SW10EXM01.gminc.local
> >
> > > Content-Type: text/plain; charset=UTF-8
> > >
> > >
> > > Hi Everyone,
> > >
> > > I am looking for workshop or class for Histology/Histotechnology that
> > > lasts anywhere from 1 week to 3 months in the U.S.
> > > Preferably in-person and not online.
> > >
> > > If you have any suggestions or contacts, please let me know.
> > >
> > > Thank You,
> > > Jessica
> > >
> > > _____________________________________________________________________
> > >
> > > Jessica Riggleman | Research Associate
> > >
> > > Globus Medical, Inc.
> > > Valley Forge Business Center
> > > 2560 General Armistead Avenue | Audubon, PA 19403
> > > Ph: (610) 930-1800 ext. 2583 <(610)%20930-1800> | Fax:
> > > website: http://www.globusmedical.com | email:
> > > jriggleman at globusmedical.com<mailto:jriggleman at globusmedical.com>
> > > An ISO 13485 Registered Company
> > >
> > > Confidentiality Note: This email is confidential and intended solely
> for
> > > the use of the individual to whom it is addressed. If you are not the
> > > intended recipient, be advised that you have received this email in
> error
> > > and that any use, dissemination, forwarding, printing, or copying of
> this
> > > email is strictly prohibited. If you have received this email in error
> > > please contact the sender. Any views or opinions presented are solely
> > those
> > > of the author and do not necessarily represent those of Globus Medical,
> > > Inc. Although this email and any attachments are believed to be free of
> > any
> > > virus or other defects which might affect any computer or IT system
> into
> > > which they are received, no responsibility is accepted by Globus
> Medical,
> > > Inc. for any loss or damage arising in any way from the receipt or use
> > > thereof.
> > >
> > > Disclaimer
> > >
> > > The information contained in this communication from the sender is
> > > confidential. It is intended solely for use by the recipient and others
> > > authorized to receive it. If you are not the recipient, you are hereby
> > > notified that any disclosure, copying, distribution or taking action in
> > > relation of the contents of this information is strictly prohibited and
> > may
> > > be unlawful.
> > >
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> > >
> > >
> > > ------------------------------
> > >
> > > Message: 2
> > > Date: Wed, 8 Feb 2017 21:33:47 +0000
> > > From: Jessica Riggleman <jriggleman at globusmedical.com>
> > > To: Esther C Peters <epeters2 at gmu.edu>
> > > Cc: "histonet at lists.utsouthwestern.edu"
> > > <histonet at lists.utsouthwestern.edu>
> > > Subject: Re: [Histonet] Looking for a Histology Workshop
> > > Message-ID:
> > > <D0AEE53F1F084340888BEB10C81DE9B295C780C0 at SW10EXM01.gminc.local
> >
> > > Content-Type: text/plain; charset=UTF-8
> > >
> > > Hi Esther,
> > >
> > > We are looking for basic training to include study design, sectioning
> raw
> > > specimens for processing (i.e., how large should my specimen be?),
> > > processing techniques (fixation, etc.), special stains, and slide
> > reading.
> > > This is not for HT/HTL exam preparation.
> > >
> > > We would prefer non-human tissues, as we typically work with large
> sheep
> > > and rabbit specimens. Only two students.
> > >
> > > Thank You,
> > > Jessica
> > >
> > >
> > > From: Esther C Peters [mailto:epeters2 at gmu.edu]
> > > Sent: Wednesday, February 08, 2017 4:18 PM
> > > To: Jessica Riggleman <jriggleman at globusmedical.com>
> > > Cc: histonet at lists.utsouthwestern.edu
> > > Subject: Re: Looking for a Histology Workshop
> > >
> > > Is this for human or non-human tissues? How many students?
> > >
> > > Esther
> > >
> > >
> > > _____________________________________________________________________
> > >
> > > Jessica Riggleman | Research Associate
> > >
> > > Globus Medical, Inc.
> > > Valley Forge Business Center
> > > 2560 General Armistead Avenue | Audubon, PA 19403
> > > Ph: (610) 930-1800 ext. 2583 <(610)%20930-1800> | Fax:
> > >
> > > Confidentiality Note: This email is confidential and intended solely
> for
> > > the use of the individual to whom it is addressed. If you are not the
> > > intended recipient, be advised that you have received this email in
> error
> > > and that any use, dissemination, forwarding, printing, or copying of
> this
> > > email is strictly prohibited. If you have received this email in error
> > > please contact the sender. Any views or opinions presented are solely
> > those
> > > of the author and do not necessarily represent those of Globus Medical,
> > > Inc. Although this email and any attachments are believed to be free of
> > any
> > > virus or other defects which might affect any computer or IT system
> into
> > > which they are received, no responsibility is accepted by Globus
> Medical,
> > > Inc. for any loss or damage arising in any way from the receipt or use
> > > thereof.
> > >
> > >
> > > ________________________________________
> > > From: Jessica Riggleman <jriggleman at globusmedical.com>
> > > Sent: Wednesday, February 8, 2017 4:12:20 PM
> > > To: Esther C Peters
> > > Cc: histonet at lists.utsouthwestern.edu
> > > Subject: RE: Looking for a Histology Workshop
> > >
> > > Hi Esther,
> > >
> > > We are looking for basic training to include study design, sectioning
> raw
> > > specimens for processing (i.e., how large should my specimen be?),
> > > processing techniques (fixation, etc.), special stains, and slide
> > reading.
> > > This is not for HT/HTL exam preparation.
> > >
> > > Thank You,
> > > Jessica
> > >
> > > ________________________________________
> > >
> > > Jessica Riggleman | Research Associate
> > >
> > > Globus Medical, Inc.
> > > Valley Forge Business Center
> > > 2560 General Armistead Avenue | Audubon, PA 19403
> > > Ph: (610) 930-1800 ext. 2583 <(610)%20930-1800> | Fax:
> > > An ISO 13485 Registered Company
> > >
> > > website | map | email
> > >
> > >
> > >
> > >
> > >
> > > Confidentiality Note: This email is confidential and intended solely
> for
> > > the use of the individual to whom it is addressed. If you are not the
> > > intended recipient, be advised that you have received this email in
> error
> > > and that any use, dissemination, forwarding, printing, or copying of
> this
> > > email is strictly prohibited. If you have received this email in error
> > > please contact the sender. Any views or opinions presented are solely
> > those
> > > of the author and do not necessarily represent those of Globus Medical,
> > > Inc. Although this email and any attachments are believed to be free of
> > any
> > > virus or other defects which might affect any computer or IT system
> into
> > > which they are received, no responsibility is accepted by Globus
> Medical,
> > > Inc. for any loss or damage arising in any way from the receipt or use
> > > thereof.
> > > From: Esther C Peters [mailto:epeters2 at gmu.edu]
> > > Sent: Wednesday, February 08, 2017 3:53 PM
> > > To: Jessica Riggleman <jriggleman at globusmedical.com>
> > > Subject: Re: Looking for a Histology Workshop
> > >
> > > Hi Jessica,
> > >
> > > It might help everyone to know exactly what you want to cover in the
> > > workshop or class. Do you need basic training, preparation for the
> HT/HTL
> > > exam, slide preparation, special stains, or slide reading? What is your
> > > goal?
> > >
> > > Esther
> > >
> > > Esther C. Peters, Ph.D.
> > > George Mason University
> > > Fairfax, VA 22030-4444
> > > ________________________________________
> > > From: Jessica Riggleman via Histonet <histonet at lists.
> utsouthwestern.edu>
> > > Sent: Wednesday, February 8, 2017 1:07:24 PM
> > > To: histonet at lists.utsouthwestern.edu
> > > Subject: [Histonet] Looking for a Histology Workshop
> > >
> > >
> > > Hi Everyone,
> > >
> > > I am looking for workshop or class for Histology/Histotechnology that
> > > lasts anywhere from 1 week to 3 months in the U.S.
> > > Preferably in-person and not online.
> > >
> > > If you have any suggestions or contacts, please let me know.
> > >
> > > Thank You,
> > > Jessica
> > >
> > > _____________________________________________________________________
> > >
> > > Jessica Riggleman | Research Associate
> > >
> > > Globus Medical, Inc.
> > > Valley Forge Business Center
> > > 2560 General Armistead Avenue | Audubon, PA 19403
> > > Ph: (610) 930-1800 ext. 2583 <(610)%20930-1800> | Fax:
> > > website: http://www.globusmedical.com | email:
> > > jriggleman at globusmedical.com<mailto:jriggleman at globusmedical.com>
> > > An ISO 13485 Registered Company
> > >
> > > Confidentiality Note: This email is confidential and intended solely
> for
> > > the use of the individual to whom it is addressed. If you are not the
> > > intended recipient, be advised that you have received this email in
> error
> > > and that any use, dissemination, forwarding, printing, or copying of
> this
> > > email is strictly prohibited. If you have received this email in error
> > > please contact the sender. Any views or opinions presented are solely
> > those
> > > of the author and do not necessarily represent those of Globus Medical,
> > > Inc. Although this email and any attachments are believed to be free of
> > any
> > > virus or other defects which might affect any computer or IT system
> into
> > > which they are received, no responsibility is accepted by Globus
> Medical,
> > > Inc. for any loss or damage arising in any way from the receipt or use
> > > thereof.
> > >
> > > Disclaimer
> > >
> > > The information contained in this communication from the sender is
> > > confidential. It is intended solely for use by the recipient and others
> > > authorized to receive it. If you are not the recipient, you are hereby
> > > notified that any disclosure, copying, distribution or taking action in
> > > relation of the contents of this information is strictly prohibited and
> > may
> > > be unlawful.
> > >
> > > This email has been scanned for viruses and malware, and may have been
> > > automatically archived by Mimecast Ltd, an innovator in Software as a
> > > Service (SaaS) for business. Providing a safer and more useful place
> for
> > > your human generated data. Specializing in; Security, archiving and
> > > compliance. To find out more visit the Mimecast website.
> > > _______________________________________________
> > > Histonet mailing list
> > > Histonet at lists.utsouthwestern.edu
> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> > >
> > > Disclaimer
> > >
> > > The information contained in this communication from the sender is
> > > confidential. It is intended solely for use by the recipient and others
> > > authorized to receive it. If you are not the recipient, you are hereby
> > > notified that any disclosure, copying, distribution or taking action in
> > > relation of the contents of this information is strictly prohibited and
> > may
> > > be unlawful.
> > >
> > > This email has been scanned for viruses and malware, and may have been
> > > automatically archived by Mimecast Ltd, an innovator in Software as a
> > > Service (SaaS) for business. Providing a safer and more useful place
> for
> > > your human generated data. Specializing in; Security, archiving and
> > > compliance. To find out more visit the Mimecast website.
> > >
> > > ------------------------------
> > >
> > > Message: 3
> > > Date: Wed, 8 Feb 2017 16:16:10 -0800
> > > From: "Jennifer" <histotech at dermspec.com>
> > > To: <histonet at lists.utsouthwestern.edu>
> > > Subject: [Histonet] hand staining immunos
> > > Message-ID: <000001d28269$b81e82f0$285b88d0$@com>
> > > Content-Type: text/plain; charset="us-ascii"
> > >
> > > Hello,
> > >
> > >
> > >
> > > My dermatopathologist wants me to start hand staining immunos for
> MITF.
> > I
> > > would really like some feedback about everyone's experiences/thoughts
> > with
> > > manually hand staining immunos and especially with MITF. Pros and
> cons? I
> > > am
> > > concerned about the amount of time this will take for a tech to babysit
> > the
> > > stain and also about the consistency of the quality in the end result.
> > > Your
> > > opinions are much appreciated.
> > >
> > >
> > >
> > > Thank you!
> > >
> > >
> > >
> > > Jennifer
> > >
> > >
> > >
> > > ------------------------------
> > >
> > > Message: 4
> > > Date: Thu, 9 Feb 2017 15:51:22 +0000 (UTC)
> > > From: Rene J Buesa <rjbuesa at yahoo.com>
> > > To: Jennifer <histotech at dermspec.com>,
> > > "histonet at lists.utsouthwestern.edu"
> > > <histonet at lists.utsouthwestern.edu>
> > > Subject: Re: [Histonet] hand staining immunos
> > > Message-ID: <267846266.1416224.1486655482834 at mail.yahoo.com>
> > > Content-Type: text/plain; charset=UTF-8
> > >
> > > You do not have to "babysit" the procedure for it has well
> defined/timed
> > > steps. You just need a timer and check the slides when required. As to
> > > procedural "dos & donts" try to get a copy of the DAKO IHC manual.Ren?
> > >
> > > On Wednesday, February 8, 2017 7:34 PM, Jennifer via Histonet <
> > > histonet at lists.utsouthwestern.edu> wrote:
> > >
> > >
> > > Hello,
> > >
> > >
> > >
> > > My dermatopathologist wants me to start hand staining immunos? for
> MITF.
> > I
> > > would really like some feedback about everyone's experiences/thoughts
> > with
> > > manually hand staining immunos and especially with MITF. Pros and
> cons? I
> > > am
> > > concerned about the amount of time this will take for a tech to babysit
> > the
> > > stain and also about the consistency of the quality in the end result.?
> > > Your
> > > opinions are much appreciated.
> > >
> > >
> > >
> > > Thank you!
> > >
> > >
> > >
> > > Jennifer
> > >
> > > _______________________________________________
> > > Histonet mailing list
> > > Histonet at lists.utsouthwestern.edu
> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> > >
> > >
> > >
> > >
> > > ------------------------------
> > >
> > > Message: 5
> > > Date: Thu, 9 Feb 2017 16:19:00 +0000
> > > From: Walter Benton <wbenton at cua.md>
> > > To: Rene J Buesa <rjbuesa at yahoo.com>, Jennifer
> > > <histotech at dermspec.com>
> > > Cc: "histonet at lists.utsouthwestern.edu"
> > > <histonet at lists.utsouthwestern.edu>
> > > Subject: Re: [Histonet] hand staining immunos
> > > Message-ID: <0952dbba122f4678aa83759b2c98d994 at MAIL01.GCU-MD.local>
> > > Content-Type: text/plain; charset="utf-8"
> > >
> > > http://www.agilent.com/cs/library/technicaloverviews/
> > > public/08002_ihc_staining_methods.pdf
> > >
> > >
> > > -----Original Message-----
> > > From: Rene J Buesa via Histonet [mailto:histonet at lists.
> > utsouthwestern.edu]
> > > Sent: Thursday, February 09, 2017 10:51 AM
> > > To: Jennifer <histotech at dermspec.com>; histonet at lists.utsouthwestern.
> edu
> > > Subject: Re: [Histonet] hand staining immunos
> > >
> > > You do not have to "babysit" the procedure for it has well
> defined/timed
> > > steps. You just need a timer and check the slides when required. As to
> > > procedural "dos & donts" try to get a copy of the DAKO IHC manual.Ren?
> > >
> > > On Wednesday, February 8, 2017 7:34 PM, Jennifer via Histonet <
> > > histonet at lists.utsouthwestern.edu> wrote:
> > >
> > >
> > > Hello,
> > >
> > >
> > >
> > > My dermatopathologist wants me to start hand staining immunos for
> MITF.
> > I
> > > would really like some feedback about everyone's experiences/thoughts
> > with
> > > manually hand staining immunos and especially with MITF. Pros and
> cons? I
> > > am concerned about the amount of time this will take for a tech to
> > babysit
> > > the stain and also about the consistency of the quality in the end
> > result.
> > > Your opinions are much appreciated.
> > >
> > >
> > >
> > > Thank you!
> > >
> > >
> > >
> > > Jennifer
> > >
> > > _______________________________________________
> > > Histonet mailing list
> > > Histonet at lists.utsouthwestern.edu
> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> > >
> > >
> > >
> > > _______________________________________________
> > > Histonet mailing list
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> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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> > > ------------------------------
> > >
> > > Subject: Digest Footer
> > >
> > > _______________________________________________
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> > > Histonet at lists.utsouthwestern.edu
> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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> > > ------------------------------
> > >
> > > End of Histonet Digest, Vol 159, Issue 8
> > > ****************************************
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>
>
> --
> Caroline Miller (mills)
> Director of Histology
> 3Scan.com
> 415 2187297 <(415)%20218-7297>
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
--
Caroline Miller (mills)
Director of Histology
3Scan.com
415 2187297
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