[Histonet] hand staining immunos

Paula Keene Pierce paula at excaliburpathology.com
Mon Feb 13 09:37:29 CST 2017 

I agree that the Sequenza would be the way to go for your lab. 
It is what I use and the cover-plates can be washed and reused. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX 405-759-7513www.excaliburpathology.com

      From: Caroline Miller via Histonet <histonet at lists.utsouthwestern.edu>
 To: Ranna Mehta <ranna0726 at gmail.com> 
Cc: "Histonet at Lists. Edu" <histonet at lists.utsouthwestern.edu>
 Sent: Monday, February 13, 2017 9:06 AM
 Subject: Re: [Histonet] hand staining immunos
   
This is a great system for semi-automated immuno. I find the results a
little cleaner than performing the technique straight on the slide. No need
to buy the whole set up (it is just a glorified timer)- just the racks and
clips are enough!

https://www.fishersci.com/shop/products/thermo-scientific-shandon-sequenza-immunostaining-center-2/p-4010433

mills

On Thu, Feb 9, 2017 at 11:32 AM, Ranna Mehta via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Hi Jennifer,
>
>  How many slides are you planning to stain in routine?  Once you will
> understand the basic, it will be a very interesting field. If you like, I
> can help you to walk through process with step by step. send me a message
> through my email.All the best and thanks.
>
> Regards
> Ranna
>
> On Thu, Feb 9, 2017 at 12:00 PM, <histonet-request at lists.
> utsouthwestern.edu>
> wrote:
>
> > Send Histonet mailing list submissions to
> >        histonet at lists.utsouthwestern.edu
> >
> > To subscribe or unsubscribe via the World Wide Web, visit
> >        http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> > or, via email, send a message with subject or body 'help' to
> >        histonet-request at lists.utsouthwestern.edu
> >
> > You can reach the person managing the list at
> >        histonet-owner at lists.utsouthwestern.edu
> >
> > When replying, please edit your Subject line so it is more specific
> > than "Re: Contents of Histonet digest..."
> >
> >
> > Today's Topics:
> >
> >    1. Looking for a Histology Workshop (Jessica Riggleman)
> >    2. Re: Looking for a Histology Workshop (Jessica Riggleman)
> >    3. hand staining immunos (Jennifer)
> >    4. Re: hand staining immunos (Rene J Buesa)
> >    5. Re: hand staining immunos (Walter Benton)
> >
> >
> > ----------------------------------------------------------------------
> >
> > Message: 1
> > Date: Wed, 8 Feb 2017 18:07:24 +0000
> > From: Jessica Riggleman <jriggleman at globusmedical.com>
> > To: "histonet at lists.utsouthwestern.edu"
> >        <histonet at lists.utsouthwestern.edu>
> > Subject: [Histonet] Looking for a Histology Workshop
> > Message-ID:
> >        <D0AEE53F1F084340888BEB10C81DE9B295C77FAC at SW10EXM01.gminc.local>
> > Content-Type: text/plain; charset=UTF-8
> >
> >
> > Hi Everyone,
> >
> > I am looking for workshop or class for Histology/Histotechnology that
> > lasts anywhere from 1 week to 3 months in the U.S.
> > Preferably in-person and not online.
> >
> > If you have any suggestions or contacts, please let me know.
> >
> > Thank You,
> > Jessica
> >
> > _____________________________________________________________________
> >
> > Jessica Riggleman | Research Associate
> >
> > Globus Medical, Inc.
> > Valley Forge Business Center
> > 2560 General Armistead Avenue | Audubon, PA 19403
> > Ph: (610) 930-1800 ext. 2583 | Fax:
> > website: http://www.globusmedical.com | email:
> > jriggleman at globusmedical.com<mailto:jriggleman at globusmedical.com>
> > An ISO 13485 Registered Company
> >
> > Confidentiality Note:  This email is confidential and intended solely for
> > the use of the individual to whom it is addressed. If you are not the
> > intended recipient, be advised that you have received this email in error
> > and that any use, dissemination, forwarding, printing, or copying of this
> > email is strictly prohibited. If you have received this email in error
> > please contact the sender. Any views or opinions presented are solely
> those
> > of the author and do not necessarily represent those of Globus Medical,
> > Inc. Although this email and any attachments are believed to be free of
> any
> > virus or other defects which might affect any computer or IT system into
> > which they are received, no responsibility is accepted by Globus Medical,
> > Inc. for any loss or damage arising in any way from the receipt or use
> > thereof.
> >
> > Disclaimer
> >
> > The information contained in this communication from the sender is
> > confidential. It is intended solely for use by the recipient and others
> > authorized to receive it. If you are not the recipient, you are hereby
> > notified that any disclosure, copying, distribution or taking action in
> > relation of the contents of this information is strictly prohibited and
> may
> > be unlawful.
> >
> > This email has been scanned for viruses and malware, and may have been
> > automatically archived by Mimecast Ltd, an innovator in Software as a
> > Service (SaaS) for business. Providing a safer and more useful place for
> > your human generated data. Specializing in; Security, archiving and
> > compliance. To find out more visit the Mimecast website.
> >
> >
> > ------------------------------
> >
> > Message: 2
> > Date: Wed, 8 Feb 2017 21:33:47 +0000
> > From: Jessica Riggleman <jriggleman at globusmedical.com>
> > To: Esther C Peters <epeters2 at gmu.edu>
> > Cc: "histonet at lists.utsouthwestern.edu"
> >        <histonet at lists.utsouthwestern.edu>
> > Subject: Re: [Histonet] Looking for a Histology Workshop
> > Message-ID:
> >        <D0AEE53F1F084340888BEB10C81DE9B295C780C0 at SW10EXM01.gminc.local>
> > Content-Type: text/plain; charset=UTF-8
> >
> > Hi Esther,
> >
> > We are looking for basic training to include study design, sectioning raw
> > specimens for processing (i.e., how large should my specimen be?),
> > processing techniques (fixation, etc.), special stains, and slide
> reading.
> > This is not for HT/HTL exam preparation.
> >
> > We would prefer non-human tissues, as we typically work with large sheep
> > and rabbit specimens. Only two students.
> >
> > Thank You,
> > Jessica
> >
> >
> > From: Esther C Peters [mailto:epeters2 at gmu.edu]
> > Sent: Wednesday, February 08, 2017 4:18 PM
> > To: Jessica Riggleman <jriggleman at globusmedical.com>
> > Cc: histonet at lists.utsouthwestern.edu
> > Subject: Re: Looking for a Histology Workshop
> >
> > Is this for human or non-human tissues? How many students?
> >
> > Esther
> >
> >
> > _____________________________________________________________________
> >
> > Jessica Riggleman  | Research Associate
> >
> > Globus Medical, Inc.
> > Valley Forge Business Center
> > 2560 General Armistead Avenue | Audubon, PA 19403
> > Ph: (610) 930-1800 ext. 2583 | Fax:
> >
> > Confidentiality Note:  This email is confidential and intended solely for
> > the use of the individual to whom it is addressed. If you are not the
> > intended recipient, be advised that you have received this email in error
> > and that any use, dissemination, forwarding, printing, or copying of this
> > email is strictly prohibited. If you have received this email in error
> > please contact the sender. Any views or opinions presented are solely
> those
> > of the author and do not necessarily represent those of Globus Medical,
> > Inc. Although this email and any attachments are believed to be free of
> any
> > virus or other defects which might affect any computer or IT system into
> > which they are received, no responsibility is accepted by Globus Medical,
> > Inc. for any loss or damage arising in any way from the receipt or use
> > thereof.
> >
> >
> > ________________________________________
> > From: Jessica Riggleman <jriggleman at globusmedical.com>
> > Sent: Wednesday, February 8, 2017 4:12:20 PM
> > To: Esther C Peters
> > Cc: histonet at lists.utsouthwestern.edu
> > Subject: RE: Looking for a Histology Workshop
> >
> > Hi Esther,
> >
> > We are looking for basic training to include study design, sectioning raw
> > specimens for processing (i.e., how large should my specimen be?),
> > processing techniques (fixation, etc.), special stains, and slide
> reading.
> > This is not for HT/HTL exam preparation.
> >
> > Thank You,
> > Jessica
> >
> > ________________________________________
> >
> > Jessica Riggleman  | Research Associate
> >
> > Globus Medical, Inc.
> > Valley Forge Business Center
> > 2560 General Armistead Avenue | Audubon, PA 19403
> > Ph: (610) 930-1800 ext. 2583 | Fax:
> > An ISO 13485 Registered Company
> >
> > website | map | email
> >
> >
> >
> >
> >
> > Confidentiality Note:  This email is confidential and intended solely for
> > the use of the individual to whom it is addressed. If you are not the
> > intended recipient, be advised that you have received this email in error
> > and that any use, dissemination, forwarding, printing, or copying of this
> > email is strictly prohibited. If you have received this email in error
> > please contact the sender. Any views or opinions presented are solely
> those
> > of the author and do not necessarily represent those of Globus Medical,
> > Inc. Although this email and any attachments are believed to be free of
> any
> > virus or other defects which might affect any computer or IT system into
> > which they are received, no responsibility is accepted by Globus Medical,
> > Inc. for any loss or damage arising in any way from the receipt or use
> > thereof.
> > From: Esther C Peters [mailto:epeters2 at gmu.edu]
> > Sent: Wednesday, February 08, 2017 3:53 PM
> > To: Jessica Riggleman <jriggleman at globusmedical.com>
> > Subject: Re: Looking for a Histology Workshop
> >
> > Hi Jessica,
> >
> > It might help everyone to know exactly what you want to cover in the
> > workshop or class. Do you need basic training, preparation for the HT/HTL
> > exam, slide preparation, special stains, or slide reading? What is your
> > goal?
> >
> > Esther
> >
> > Esther C. Peters, Ph.D.
> > George Mason University
> > Fairfax, VA 22030-4444
> > ________________________________________
> > From: Jessica Riggleman via Histonet <histonet at lists.utsouthwestern.edu>
> > Sent: Wednesday, February 8, 2017 1:07:24 PM
> > To: histonet at lists.utsouthwestern.edu
> > Subject: [Histonet] Looking for a Histology Workshop
> >
> >
> > Hi Everyone,
> >
> > I am looking for workshop or class for Histology/Histotechnology that
> > lasts anywhere from 1 week to 3 months in the U.S.
> > Preferably in-person and not online.
> >
> > If you have any suggestions or contacts, please let me know.
> >
> > Thank You,
> > Jessica
> >
> > _____________________________________________________________________
> >
> > Jessica Riggleman | Research Associate
> >
> > Globus Medical, Inc.
> > Valley Forge Business Center
> > 2560 General Armistead Avenue | Audubon, PA 19403
> > Ph: (610) 930-1800 ext. 2583 | Fax:
> > website: http://www.globusmedical.com | email:
> > jriggleman at globusmedical.com<mailto:jriggleman at globusmedical.com>
> > An ISO 13485 Registered Company
> >
> > Confidentiality Note:  This email is confidential and intended solely for
> > the use of the individual to whom it is addressed. If you are not the
> > intended recipient, be advised that you have received this email in error
> > and that any use, dissemination, forwarding, printing, or copying of this
> > email is strictly prohibited. If you have received this email in error
> > please contact the sender. Any views or opinions presented are solely
> those
> > of the author and do not necessarily represent those of Globus Medical,
> > Inc. Although this email and any attachments are believed to be free of
> any
> > virus or other defects which might affect any computer or IT system into
> > which they are received, no responsibility is accepted by Globus Medical,
> > Inc. for any loss or damage arising in any way from the receipt or use
> > thereof.
> >
> > Disclaimer
> >
> > The information contained in this communication from the sender is
> > confidential. It is intended solely for use by the recipient and others
> > authorized to receive it. If you are not the recipient, you are hereby
> > notified that any disclosure, copying, distribution or taking action in
> > relation of the contents of this information is strictly prohibited and
> may
> > be unlawful.
> >
> > This email has been scanned for viruses and malware, and may have been
> > automatically archived by Mimecast Ltd, an innovator in Software as a
> > Service (SaaS) for business. Providing a safer and more useful place for
> > your human generated data. Specializing in; Security, archiving and
> > compliance. To find out more visit the Mimecast website.
> > _______________________________________________
> > Histonet mailing list
> > Histonet at lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> > Disclaimer
> >
> > The information contained in this communication from the sender is
> > confidential. It is intended solely for use by the recipient and others
> > authorized to receive it. If you are not the recipient, you are hereby
> > notified that any disclosure, copying, distribution or taking action in
> > relation of the contents of this information is strictly prohibited and
> may
> > be unlawful.
> >
> > This email has been scanned for viruses and malware, and may have been
> > automatically archived by Mimecast Ltd, an innovator in Software as a
> > Service (SaaS) for business. Providing a safer and more useful place for
> > your human generated data. Specializing in; Security, archiving and
> > compliance. To find out more visit the Mimecast website.
> >
> > ------------------------------
> >
> > Message: 3
> > Date: Wed, 8 Feb 2017 16:16:10 -0800
> > From: "Jennifer" <histotech at dermspec.com>
> > To: <histonet at lists.utsouthwestern.edu>
> > Subject: [Histonet] hand staining immunos
> > Message-ID: <000001d28269$b81e82f0$285b88d0$@com>
> > Content-Type: text/plain;      charset="us-ascii"
> >
> > Hello,
> >
> >
> >
> > My dermatopathologist wants me to start hand staining immunos  for MITF.
> I
> > would really like some feedback about everyone's experiences/thoughts
> with
> > manually hand staining immunos and especially with MITF. Pros and cons? I
> > am
> > concerned about the amount of time this will take for a tech to babysit
> the
> > stain and also about the consistency of the quality in the end result.
> > Your
> > opinions are much appreciated.
> >
> >
> >
> > Thank you!
> >
> >
> >
> > Jennifer
> >
> >
> >
> > ------------------------------
> >
> > Message: 4
> > Date: Thu, 9 Feb 2017 15:51:22 +0000 (UTC)
> > From: Rene J Buesa <rjbuesa at yahoo.com>
> > To: Jennifer <histotech at dermspec.com>,
> >        "histonet at lists.utsouthwestern.edu"
> >        <histonet at lists.utsouthwestern.edu>
> > Subject: Re: [Histonet] hand staining immunos
> > Message-ID: <267846266.1416224.1486655482834 at mail.yahoo.com>
> > Content-Type: text/plain; charset=UTF-8
> >
> > You do not have to "babysit" the procedure for it has well defined/timed
> > steps. You just need a timer and check the slides when required. As to
> > procedural "dos & donts" try to get a copy of the DAKO IHC manual.Ren?
> >
> >    On Wednesday, February 8, 2017 7:34 PM, Jennifer via Histonet <
> > histonet at lists.utsouthwestern.edu> wrote:
> >
> >
> >  Hello,
> >
> >
> >
> > My dermatopathologist wants me to start hand staining immunos? for MITF.
> I
> > would really like some feedback about everyone's experiences/thoughts
> with
> > manually hand staining immunos and especially with MITF. Pros and cons? I
> > am
> > concerned about the amount of time this will take for a tech to babysit
> the
> > stain and also about the consistency of the quality in the end result.?
> > Your
> > opinions are much appreciated.
> >
> >
> >
> > Thank you!
> >
> >
> >
> > Jennifer
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet at lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
> >
> >
> > ------------------------------
> >
> > Message: 5
> > Date: Thu, 9 Feb 2017 16:19:00 +0000
> > From: Walter Benton <wbenton at cua.md>
> > To: Rene J Buesa <rjbuesa at yahoo.com>, Jennifer
> >        <histotech at dermspec.com>
> > Cc: "histonet at lists.utsouthwestern.edu"
> >        <histonet at lists.utsouthwestern.edu>
> > Subject: Re: [Histonet] hand staining immunos
> > Message-ID: <0952dbba122f4678aa83759b2c98d994 at MAIL01.GCU-MD.local>
> > Content-Type: text/plain; charset="utf-8"
> >
> > http://www.agilent.com/cs/library/technicaloverviews/
> > public/08002_ihc_staining_methods.pdf
> >
> >
> > -----Original Message-----
> > From: Rene J Buesa via Histonet [mailto:histonet at lists.
> utsouthwestern.edu]
> > Sent: Thursday, February 09, 2017 10:51 AM
> > To: Jennifer <histotech at dermspec.com>; histonet at lists.utsouthwestern.edu
> > Subject: Re: [Histonet] hand staining immunos
> >
> > You do not have to "babysit" the procedure for it has well defined/timed
> > steps. You just need a timer and check the slides when required. As to
> > procedural "dos & donts" try to get a copy of the DAKO IHC manual.Ren?
> >
> >    On Wednesday, February 8, 2017 7:34 PM, Jennifer via Histonet <
> > histonet at lists.utsouthwestern.edu> wrote:
> >
> >
> >  Hello,
> >
> >
> >
> > My dermatopathologist wants me to start hand staining immunos  for MITF.
> I
> > would really like some feedback about everyone's experiences/thoughts
> with
> > manually hand staining immunos and especially with MITF. Pros and cons? I
> > am concerned about the amount of time this will take for a tech to
> babysit
> > the stain and also about the consistency of the quality in the end
> result.
> > Your opinions are much appreciated.
> >
> >
> >
> > Thank you!
> >
> >
> >
> > Jennifer
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet at lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet at lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> > CONFIDENTIALITY NOTICE: The information contained in this electronic
> > message is intended solely for the personal and confidential use of the
> > designated recipient(s) named above and may contain information that is
> > protected from disclosure under applicable law. If you are not the
> intended
> > recipient, or the employee or agent responsible for delivering it to the
> > intended recipient, you are hereby notified that any dissemination,
> > distribution or copying of this transmission is strictly prohibited. If
> you
> > have received this transmission in error, please notify the transmitting
> > person/department immediately by email or telephone (410) 581-5881 and
> > delete the message without making a copy.
> >
> > ------------------------------
> >
> > Subject: Digest Footer
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet at lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> > ------------------------------
> >
> > End of Histonet Digest, Vol 159, Issue 8
> > ****************************************
> >
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



-- 
Caroline Miller (mills)
Director of Histology
3Scan.com
415 2187297
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

More information about the Histonet mailing list