[Histonet] Handstaining IHC for MITC

Terri Braud tbraud at holyredeemer.com
Thu Feb 9 13:02:13 CST 2017

Coming from an ancient histology tech who did IHC back in the dark ages, before automation, hand staining is a piece of cake.
A couple of hints:
Use a "pap" pen to circle your tissue on the slide to form a barrier to keep your reagents puddled in a concentrated spot and to minimize reagent use.
Use a plastic 100 slide box as an incubation chamber.  Place dampened strips of paper towels in the bottom to make a moist chamber, and lay the slides flat across the filing slots.  Keep the lid closed between steps.
Use a reliable kit and pre-dilutes to make life easier.
Best of luck.

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal

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