[Histonet] Immunohistochemistry ghosting

[Duddey, Aimee]

When we saw this happening it was slide related, especially with it happening across all platforms.  We use Cardinal Healthcare SuperFrost Plus and haven't had an issue.  Every time we have tried another brand we have seen this happen to some degree. 

Aimee M. Duddey, MLT(ASCP)
Assistant Director of Laboratory - Pathology
FH Moore Regional Hospital
910-715-5286 office
910715-1944 fax


-----Original Message-----
From: Robyn L. Vazquez via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Thursday, February 09, 2017 7:42 PM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Immunohistochemistry ghosting

Hello,
We have been experiencing problems with ghosting (inconsistent and/or no staining) mainly affecting our control area which is located at the bottom of the slide.  Random antibodies markers are being affected. We were using Fisher Scientific then TruBond slides. We are using Ventana Ultras X4 and XT in which it is occurring on all machines. Ventana has come in to troubleshoot, in which we have not reached a solution.

Any suggestions would be greatly appreciated

Robyn L. Vazquez
Kaiser Permanente
Portland, Oregon
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