[Histonet] Immunohistochemistry ghosting

Miranda Giorgi mgiorgi at incdx.com
Thu Feb 9 19:14:27 CST 2017

Hi Robyn,

We had the same issue last year and it came down to slide type for us.  We were using a charged slide from Cancer Diagnostics, but have since switched to Mercedes Platinum Line Starfrost slides.  We have not had any issues with "ghost tissue" since then.

Miranda Giorgi, HTL (ASCP)cm
Histology Manager
mgiorgi at incdx.com

-----Original Message-----
From: Robyn L. Vazquez via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Thursday, February 09, 2017 4:42 PM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Immunohistochemistry ghosting

We have been experiencing problems with ghosting (inconsistent and/or no staining) mainly affecting our control area which is located at the bottom of the slide.  Random antibodies markers are being affected. We were using Fisher Scientific then TruBond slides. We are using Ventana Ultras X4 and XT in which it is occurring on all machines. Ventana has come in to troubleshoot, in which we have not reached a solution.

Any suggestions would be greatly appreciated

Robyn L. Vazquez
Kaiser Permanente
Portland, Oregon
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