[Histonet] Immunohistochemistry ghosting

[Robyn L. Vazquez]

Hello,
We have been experiencing problems with ghosting (inconsistent and/or no staining) mainly affecting our control area which is located at the bottom of the slide.  Random antibodies markers are being affected. We were using Fisher Scientific then TruBond slides. We are using Ventana Ultras X4 and XT in which it is occurring on all machines. Ventana has come in to troubleshoot, in which we have not reached a solution.

Any suggestions would be greatly appreciated

Robyn L. Vazquez
Kaiser Permanente
Portland, Oregon
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