[Histonet] IHC

[Dessasau III, Evan]

Hi again Ms. Horst,  For some reason I did not see all the replies from Mr. Cooper.  But wait before your order the Superfrost slides.  If you are not familiar with them they are good as Mr. Cooper says but they can be pricey.  We use a great guy at PathSUPPLY, who is cc'd here, that can get you a comparable (if not the same) slide at a lower price point.  Also, we use slides packaged for PathSupply that are a good price point and excellent quality esp. the Bright White slide is comparable to the Superfrost but at a lower price point.  Also, sorry I would suggest you heat them before you send them and communicate with them like someone else said.  I always ask how they want them treated before I send the sections.
E-van

-----Original Message-----
From: Emily Horst via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Thursday, August 10, 2017 6:18 PM
To: histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC

Thanks everyone for all your suggestions! You've been very helpful!

On Thu, Aug 10, 2017 at 6:16 PM Cooper, Brian <bcooper at chla.usc.edu> wrote:

> See if you can get a sample of slides from Thermo Fisher.  We use the
> Superfrost Plus slides, and tissue loss just isn’t one of the problems
> we ever have here.  And like Tim Morken has said in his response,
> ensure there’s no trapped water under the section.
>
>
>
>
> https://www.fishersci.com/shop/products/shandon-colorfrost-plus-slides
> /6776214?searchHijack=true&searchTerm=6776214&searchType=RAPID&matched
> CatNo=6776214CS
>
>
>
> Thanks,
>
>
>
> Brian
>
>
>
> *From:* Emily Horst [mailto:mashalsmom at gmail.com]
> *Sent:* Thursday, August 10, 2017 2:54 PM
>
>
> *To:* Cooper, Brian
> *Subject:* Re: [Histonet] IHC (EXTERNAL EMAIL)
>
>
>
> They are ones that were prepackaged and sent to us from Dianon (Dianon
> is not the lab I'm talking about). I have no idea what/who the slides
> are. I just ordered new charged slides from Mercedes Medical and all
> my invoice says is 45 degree, white. I haven't received them yet so I
> don't have a box with info on it.
>
>
>
> It's a problem we've been having for awhile.
>
>
>
> On Thu, Aug 10, 2017 at 5:49 PM Cooper, Brian <bcooper at chla.usc.edu>
> wrote:
>
> So I worked at a reference lab for yeeeeeeeeeears and years and years,
> and I can tell you that not all charged slides are created equally.
> What type of slides are you using?
>
>
>
> Thanks,
>
>
>
> Brian
>
>
>
> *From:* Emily Horst [mailto:mashalsmom at gmail.com]
> *Sent:* Thursday, August 10, 2017 2:47 PM
> *To:* Cooper, Brian
> *Subject:* Re: [Histonet] IHC (EXTERNAL EMAIL)
>
>
>
> No I am not. I air dry them and then package them up.
>
>
>
> On Thu, Aug 10, 2017 at 5:46 PM Cooper, Brian <bcooper at chla.usc.edu>
> wrote:
>
> Are you baking the slides before sending them out?  How long, and what
> temperature?
>
> Thanks,
>
> Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of
> Pathology and Laboratory Medicine Children's Hospital Los Angeles
> 4650 Sunset Blvd MS#43- Los Angeles, CA 90027
> Ph: 323.361.3357     Pager: 213-209-0184
> bcooper at chla.usc.edu
>
> -----Original Message-----
> From: Emily Horst via Histonet
> [mailto:histonet at lists.utsouthwestern.edu]
> Sent: Thursday, August 10, 2017 2:42 PM
> To: histonet at lists.utsouthwestern.edu
> Subject: [Histonet] IHC (EXTERNAL EMAIL)
>
> Hi everyone,
>
> We send our IHCs to an outside lab. I send them the unstained slides.
> I cut them at 4 microns and put them on charged slides. We are having
> a lot of issues with the receiving lab saying the tissue is falling
> off. Their supervisor says the sections are too thick (remember 4
> microns) and my pathologist says they are not too thick. Any
> suggestions for resolving this issue with the receiving lab?
>
> Thanks!
> --
> Emily Horst, HT (ASCP) Office manager Interventional Pathology of Ohio
> Kettering, Ohio 937.297.0028
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> --
>
> Emily Horst, HT (ASCP) Office manager Interventional Pathology of Ohio
> Kettering, Ohio 937.297.0028
>
> CONFIDENTIALITY NOTICE: This e-mail message, including any
> attachments, is for the sole use of the intended recipient(s) and may
> contain confidential or legally privileged information. Any
> unauthorized review, use, disclosure or distribution is prohibited. If
> you are not the intended recipient, please contact the sender by reply
> e-mail and destroy all copies of this original message.
>
> --
>
> Emily Horst, HT (ASCP) Office manager Interventional Pathology of Ohio
> Kettering, Ohio 937.297.0028
>
> CONFIDENTIALITY NOTICE: This e-mail message, including any
> attachments, is for the sole use of the intended recipient(s) and may
> contain confidential or legally privileged information. Any
> unauthorized review, use, disclosure or distribution is prohibited. If
> you are not the intended recipient, please contact the sender by reply
> e-mail and destroy all copies of this original message.
>
--
Emily Horst, HT (ASCP) Office manager Interventional Pathology of Ohio Kettering, Ohio 937.297.0028 _______________________________________________
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