[Histonet] IHC

[Emily Horst]

Thanks everyone for all your suggestions! You've been very helpful!

On Thu, Aug 10, 2017 at 6:16 PM Cooper, Brian <bcooper at chla.usc.edu> wrote:

> See if you can get a sample of slides from Thermo Fisher.  We use the
> Superfrost Plus slides, and tissue loss just isn’t one of the problems we
> ever have here.  And like Tim Morken has said in his response, ensure
> there’s no trapped water under the section.
>
>
>
>
> https://www.fishersci.com/shop/products/shandon-colorfrost-plus-slides/6776214?searchHijack=true&searchTerm=6776214&searchType=RAPID&matchedCatNo=6776214CS
>
>
>
> Thanks,
>
>
>
> Brian
>
>
>
> *From:* Emily Horst [mailto:mashalsmom at gmail.com]
> *Sent:* Thursday, August 10, 2017 2:54 PM
>
>
> *To:* Cooper, Brian
> *Subject:* Re: [Histonet] IHC (EXTERNAL EMAIL)
>
>
>
> They are ones that were prepackaged and sent to us from Dianon (Dianon is
> not the lab I'm talking about). I have no idea what/who the slides are. I
> just ordered new charged slides from Mercedes Medical and all my invoice
> says is 45 degree, white. I haven't received them yet so I don't have a box
> with info on it.
>
>
>
> It's a problem we've been having for awhile.
>
>
>
> On Thu, Aug 10, 2017 at 5:49 PM Cooper, Brian <bcooper at chla.usc.edu>
> wrote:
>
> So I worked at a reference lab for yeeeeeeeeeears and years and years, and
> I can tell you that not all charged slides are created equally.  What type
> of slides are you using?
>
>
>
> Thanks,
>
>
>
> Brian
>
>
>
> *From:* Emily Horst [mailto:mashalsmom at gmail.com]
> *Sent:* Thursday, August 10, 2017 2:47 PM
> *To:* Cooper, Brian
> *Subject:* Re: [Histonet] IHC (EXTERNAL EMAIL)
>
>
>
> No I am not. I air dry them and then package them up.
>
>
>
> On Thu, Aug 10, 2017 at 5:46 PM Cooper, Brian <bcooper at chla.usc.edu>
> wrote:
>
> Are you baking the slides before sending them out?  How long, and what
> temperature?
>
> Thanks,
>
> Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
> Department of Pathology and Laboratory Medicine
> Children's Hospital Los Angeles
> 4650 Sunset Blvd MS#43- Los Angeles, CA 90027
> Ph: 323.361.3357     Pager: 213-209-0184
> bcooper at chla.usc.edu
>
> -----Original Message-----
> From: Emily Horst via Histonet [mailto:histonet at lists.utsouthwestern.edu]
> Sent: Thursday, August 10, 2017 2:42 PM
> To: histonet at lists.utsouthwestern.edu
> Subject: [Histonet] IHC (EXTERNAL EMAIL)
>
> Hi everyone,
>
> We send our IHCs to an outside lab. I send them the unstained slides. I
> cut them at 4 microns and put them on charged slides. We are having a lot
> of issues with the receiving lab saying the tissue is falling off. Their
> supervisor says the sections are too thick (remember 4 microns) and my
> pathologist says they are not too thick. Any suggestions for resolving this
> issue with the receiving lab?
>
> Thanks!
> --
> Emily Horst, HT (ASCP) Office manager Interventional Pathology of Ohio
> Kettering, Ohio 937.297.0028 _______________________________________________
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> Histonet at lists.utsouthwestern.edu
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> --
>
> Emily Horst, HT (ASCP) Office manager Interventional Pathology of Ohio
> Kettering, Ohio 937.297.0028
>
> CONFIDENTIALITY NOTICE: This e-mail message, including any attachments,
> is for the sole use of the intended recipient(s) and may contain
> confidential
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> --
>
> Emily Horst, HT (ASCP) Office manager Interventional Pathology of Ohio
> Kettering, Ohio 937.297.0028
>
> CONFIDENTIALITY NOTICE: This e-mail message, including any attachments,
> is for the sole use of the intended recipient(s) and may contain
> confidential
> or legally privileged information. Any unauthorized review, use, disclosure
> or distribution is prohibited. If you are not the intended recipient,
> please
> contact the sender by reply e-mail and destroy all copies of this original
> message.
>
-- 
Emily Horst, HT (ASCP) Office manager Interventional Pathology of Ohio
Kettering, Ohio 937.297.0028