[Histonet] IHC

[Dessasau III, Evan]

Hi Emily , we do the same thing.  It could be the slides but I think it has more to do  with how you treat the sections after mounting.  The dry time and heat time.  I also think it has more to do with technique in the IHC lab.  Brains, decal, and fatty tissues get more dry time.  All sections get 1 hr. heat at 60C .  if they dry overnight 15min heat seems to be fine for us.
E-van

-----Original Message-----
From: Emily Horst via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Thursday, August 10, 2017 5:42 PM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] IHC

Hi everyone,

We send our IHCs to an outside lab. I send them the unstained slides. I cut them at 4 microns and put them on charged slides. We are having a lot of issues with the receiving lab saying the tissue is falling off. Their supervisor says the sections are too thick (remember 4 microns) and my pathologist says they are not too thick. Any suggestions for resolving this issue with the receiving lab?

Thanks!
--
Emily Horst, HT (ASCP) Office manager Interventional Pathology of Ohio Kettering, Ohio 937.297.0028 _______________________________________________
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