[Histonet] No Ribbon

Tue Apr 11 13:18:36 CDT 2017

Point taken, thanks Elizabeth.

My clinical days are long gone, but that is an important consideration.

yours,
mills

On Tue, Apr 11, 2017 at 11:13 AM, Elizabeth Chlipala <liz at premierlab.com>
wrote:

> I might get some individuals upset but we really need to watch what we do
> in the histology lab and how that effects the precision of our process.
> When you breath on the block you are basically generating a thicker
> section, it's not a good practice to keep.  Trust me I used to do this in
> the past.  Thicker sections especially for IHC will generate a more intense
> stain, most individuals may think that that should not be an issue but it
> is - in the clinical setting especially when you are staining for
> therapeutic markers such as Her2 and PD-L1 - 510K cleared kits for these
> recommend specific section thickness settings and in the research setting
> if you are running any type of analysis on a batch of samples.  If your
> microtome is set at 4 microns and you are breathing on the block you are
> not cutting at 4 microns, you are cutting thicker than that and a thicker
> section will increase staining intensity and therefore alter the results.
>
> Just my two cents.
>
> Liz
>
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
> Premier Laboratory, LLC
> PO Box 18592
> Boulder, CO 80308
> (303) 682-3949 office
> (303) 682-9060 fax
> (303) 881-0763 cell
> liz at premierlab.com
> www.premierlab.com
>
> Ship to Address:
>
> Premier Laboratory, LLC
> 1567 Skyway Drive, Unit E
> Longmont, CO 80504
>
>
> -----Original Message-----
> From: Caroline Miller via Histonet [mailto:histonet at lists.
> utsouthwestern.edu]
> Sent: Tuesday, April 11, 2017 11:59 AM
> To: Paula Keene Pierce
> Cc: Histonet Histonet
> Subject: Re: [Histonet] No Ribbon
>
> Yes, +1 to Paula, also:
> - I often found they are too sharp (!!) to ribbon so a gentle wipe (in the
> direction of the bevel) with a kimwipe helps
> - Also 'huffing' slightly on the block will warm the paraffin and both
> smooth out the wrinkles as well as make the sections stick together
>
> :)
>
> mills
>
> On Tue, Apr 11, 2017 at 10:37 AM, Paula Keene Pierce via Histonet <
> histonet at lists.utsouthwestern.edu> wrote:
>
> > Hi,
> > the blades are packed with a tiny, bit of oil. This can cause you to
> > not obtain a ribbon.
> > Try gently cleaning with xylene, followed by alcohol before
> > sectioning. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur
> > Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH
> > 405-759-3953FAX 405-759-7513www.excaliburpathology.com
> >
> >       From: Gareth Davis via Histonet
> > <histonet at lists.utsouthwestern.edu>
> >  To: "Histonet at lists.utsouthwestern.edu" <histonet at lists.
> > utsouthwestern.edu>
> >  Sent: Tuesday, April 11, 2017 11:55 AM
> >  Subject: [Histonet] No Ribbon
> >
> > I feel like I should be able to figure this one out, but I have been
> > having trouble with getting a ribbon for a while now.  I have a Leica
> > Microtome
> > RM2125 and I use either a AccuEdge blade (I get one block to cut then
> > it starts to compress) or a StatCut blade (sections do not stick
> > together at all), both High Profile.  The angle is about 4 microns.  I
> > change the angle a lot to see if that makes a difference and it
> > doesn't.  There are so many factors that could be the issue, I think
> > lack of humidity may be the main one. So, suggestions please!!
> >
> > --
> > Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology
> > Yuma, AZ 85364
> > 928-248-5259
> > _______________________________________________
> > Histonet mailing list
> > Histonet at lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
> >
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> >
>
>
>
> --
> Caroline Miller (mills)
> Director of Histology
> 3Scan.com
> 415 2187297
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-- 
Caroline Miller (mills)
Director of Histology
3Scan.com
415 2187297