[Histonet] No Ribbon

[Elizabeth Chlipala]

I might get some individuals upset but we really need to watch what we do in the histology lab and how that effects the precision of our process.  When you breath on the block you are basically generating a thicker section, it's not a good practice to keep.  Trust me I used to do this in the past.  Thicker sections especially for IHC will generate a more intense stain, most individuals may think that that should not be an issue but it is - in the clinical setting especially when you are staining for therapeutic markers such as Her2 and PD-L1 - 510K cleared kits for these recommend specific section thickness settings and in the research setting if you are running any type of analysis on a batch of samples.  If your microtome is set at 4 microns and you are breathing on the block you are not cutting at 4 microns, you are cutting thicker than that and a thicker section will increase staining intensity and therefore alter the results.

Just my two cents.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
liz at premierlab.com
www.premierlab.com

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504


-----Original Message-----
From: Caroline Miller via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Tuesday, April 11, 2017 11:59 AM
To: Paula Keene Pierce
Cc: Histonet Histonet
Subject: Re: [Histonet] No Ribbon

Yes, +1 to Paula, also:
- I often found they are too sharp (!!) to ribbon so a gentle wipe (in the direction of the bevel) with a kimwipe helps
- Also 'huffing' slightly on the block will warm the paraffin and both smooth out the wrinkles as well as make the sections stick together

:)

mills

On Tue, Apr 11, 2017 at 10:37 AM, Paula Keene Pierce via Histonet < histonet at lists.utsouthwestern.edu> wrote:

> Hi,
> the blades are packed with a tiny, bit of oil. This can cause you to 
> not obtain a ribbon.
> Try gently cleaning with xylene, followed by alcohol before 
> sectioning. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur 
> Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 
> 405-759-3953FAX 405-759-7513www.excaliburpathology.com
>
>       From: Gareth Davis via Histonet 
> <histonet at lists.utsouthwestern.edu>
>  To: "Histonet at lists.utsouthwestern.edu" <histonet at lists.
> utsouthwestern.edu>
>  Sent: Tuesday, April 11, 2017 11:55 AM
>  Subject: [Histonet] No Ribbon
>
> I feel like I should be able to figure this one out, but I have been 
> having trouble with getting a ribbon for a while now.  I have a Leica 
> Microtome
> RM2125 and I use either a AccuEdge blade (I get one block to cut then 
> it starts to compress) or a StatCut blade (sections do not stick 
> together at all), both High Profile.  The angle is about 4 microns.  I 
> change the angle a lot to see if that makes a difference and it 
> doesn't.  There are so many factors that could be the issue, I think 
> lack of humidity may be the main one. So, suggestions please!!
>
> --
> Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology 
> Yuma, AZ 85364
> 928-248-5259
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



--
Caroline Miller (mills)
Director of Histology
3Scan.com
415 2187297
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

______________________________________________________________________
This email has been scanned by the Symantec Email Security.cloud service.
For more information please visit http://www.symanteccloud.com
______________________________________________________________________