[Histonet] No Ribbon

[Caroline Miller]

Yes, +1 to Paula, also:
- I often found they are too sharp (!!) to ribbon so a gentle wipe (in the
direction of the bevel) with a kimwipe helps
- Also 'huffing' slightly on the block will warm the paraffin and both
smooth out the wrinkles as well as make the sections stick together

:)

mills

On Tue, Apr 11, 2017 at 10:37 AM, Paula Keene Pierce via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Hi,
> the blades are packed with a tiny, bit of oil. This can cause you to not
> obtain a ribbon.
> Try gently cleaning with xylene, followed by alcohol before
> sectioning. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur
> Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX
> 405-759-7513www.excaliburpathology.com
>
>       From: Gareth Davis via Histonet <histonet at lists.utsouthwestern.edu>
>  To: "Histonet at lists.utsouthwestern.edu" <histonet at lists.
> utsouthwestern.edu>
>  Sent: Tuesday, April 11, 2017 11:55 AM
>  Subject: [Histonet] No Ribbon
>
> I feel like I should be able to figure this one out, but I have been having
> trouble with getting a ribbon for a while now.  I have a Leica Microtome
> RM2125 and I use either a AccuEdge blade (I get one block to cut then it
> starts to compress) or a StatCut blade (sections do not stick together at
> all), both High Profile.  The angle is about 4 microns.  I change the angle
> a lot to see if that makes a difference and it doesn't.  There are so many
> factors that could be the issue, I think lack of humidity may be the main
> one. So, suggestions please!!
>
> --
> Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm
> Yuma Gastroenterology
> Yuma, AZ 85364
> 928-248-5259
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>
>
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-- 
Caroline Miller (mills)
Director of Histology
3Scan.com
415 2187297