[Histonet] No Ribbon

[Paula Keene Pierce]

Hi,
the blades are packed with a tiny, bit of oil. This can cause you to not obtain a ribbon.
Try gently cleaning with xylene, followed by alcohol before sectioning. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX 405-759-7513www.excaliburpathology.com

      From: Gareth Davis via Histonet <histonet at lists.utsouthwestern.edu>
 To: "Histonet at lists.utsouthwestern.edu" <histonet at lists.utsouthwestern.edu> 
 Sent: Tuesday, April 11, 2017 11:55 AM
 Subject: [Histonet] No Ribbon
   
I feel like I should be able to figure this one out, but I have been having
trouble with getting a ribbon for a while now.  I have a Leica Microtome
RM2125 and I use either a AccuEdge blade (I get one block to cut then it
starts to compress) or a StatCut blade (sections do not stick together at
all), both High Profile.  The angle is about 4 microns.  I change the angle
a lot to see if that makes a difference and it doesn't.  There are so many
factors that could be the issue, I think lack of humidity may be the main
one. So, suggestions please!!

-- 
Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm
Yuma Gastroenterology
Yuma, AZ 85364
928-248-5259
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