[Histonet] Buffered formalin substitution
j.benavides at eae.csic.es
Fri Sep 30 06:35:59 CDT 2016
The health officer of our institute has risen (again!) the issue of
substituting buffered formalin for some other less hazardous fixative .
I would like to know you opinion, and experience, in such matter. Is
it even possible? Have you successfully done it? In that case, which
samples you normally handle? We are a research institute, doing
several ruminant PMs a week, so big chunks of tissue to fix. Then, we
normally do IHCs with a variety of antibodies, depending on the
project. My worries are that, in case buffered formalin could be
substituted, you can never be sure about IHC and maybe antibodies that
were working before stop doing it.
Very many thanks for your thoughts and time in this issue. Greatly
More information about the Histonet