[Histonet] buffered zn-formalin recipe needed

[Caroline Miller]

Histonet archives FTW!

Also IHC world is your friend:
http://www.ihcworld.com/_protocols/histology/zinc_fixative.htm

(and whilst i am here I shall also recommend
http://stainsfile.info/StainsFile/jindex.html as a great friend too)!

mills

On Wed, Sep 28, 2016 at 10:41 AM, Amos Brooks via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Hi Johanna,
>      The Histonet Archives are your friend. In 2009, Gayle Callis posted a
> great formulation that being the neo-Luddite that I am I printed and have a
> copy of here for just such an occasion...
>
> http://lists.utsouthwestern.edu/mailman/htdig/histonet/
> 2009-October/046985.html
>
> Zinc Fixative (JB Fixative or ZSF)
>
> 0.1M Tris Buffer, pH 7.4
>       Tris Base -------------------------------- 12.1 g (TRIZMA)
>       1N HCL ----------------------------------- 81.5 ml
>       Distilled water -------------------------- 900 ml
>
>       Mix to dissolve. Adjust pH to 7.4
> Zinc Fixative
>       Calcium Acetate ---------------------- 0.5 g
>       Zinc Acetate -------------------------- 5.0 g
>       Zinc Chloride -------------------------- 5.0 g
>       0.1M Tris Buffer made above ------ 1000 ml
> Mix to dissolve. The final pH will be approximately 6.5-7.0. Do not
> readjust
> the pH, as this will cause the zinc to come out of solution. Store Zinc
> Fixative at room temperature. Fix tissues for 24 to 48 hours. Fixation
> longer than 48 hours may make the tissue brittle and difficult to cut.
>
> Good Luck,
>
> Amos
>
>
>
>
> On Wed, Sep 28, 2016 at 12:33 PM, <histonet-request at lists.
> utsouthwestern.edu
> > wrote:
>
> > Message: 6
> > Date: Tue, 27 Sep 2016 18:21:12 +0000
> > From: "Preiszner, Johanna" <PREISZNE at mail.etsu.edu>
> > To: "histonet at lists.utsouthwestern.edu"
> >         <histonet at lists.utsouthwestern.edu>
> > Subject: [Histonet] buffered zn-formalin recipe needed
> > Message-ID:
> >         <MWHPR07MB3134BF1F305A0C29CFD682639CCC0 at MWHPR07MB3134.
> > namprd07.prod.outlook.com>
> >
> > Content-Type: text/plain; charset="iso-8859-1"
> >
> > Hi,
> >
> >
> > For the life of me I can't locate a recipe for buffered zn-formalin, nor
> > online or off.
> >
> >
> > Could someone please help us out?
> >
> >
> > Thanks,
> >
> > Hanna Preiszner
> >
> > ETSU/QCOM
> >
> _______________________________________________
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> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



-- 
Caroline Miller (mills)
Director of Histology
3Scan.com
415 2187297