[Histonet] Buffered formalin substitution

Rene J Buesa rjbuesa at yahoo.com
Fri Sep 30 09:29:28 CDT 2016

Julio:Unfortunately NBF is the OVERALL best fixative there is. ANY substitute will be good for some things and not that good for others. Under those circumstances what to do? Simply use LESS amounts of formalin, do it safely keeping to a minimum its exposure.Under separate cover I am sending you 2 articles I published on the subject.René 

    On Friday, September 30, 2016 8:04 AM, Julio Benavides via Histonet <histonet at lists.utsouthwestern.edu> wrote:

 Hi there,

The health officer of our institute has risen (again!) the issue of  
substituting buffered formalin for some other less hazardous fixative .

I would like to know you opinion, and experience, in such matter. Is  
it even possible? Have you successfully done it? In that case, which  
samples you normally handle? We are a research institute, doing  
several ruminant PMs a week, so big chunks of tissue to fix. Then, we  
normally do IHCs with a variety of antibodies, depending on the  
project.  My worries are that, in case buffered formalin could be  
substituted, you can never be sure about IHC and maybe antibodies that  
were working before stop doing it.

Very many thanks for your thoughts and time in this issue. Greatly  



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