[Histonet] Question on Sanderson Rapid Bone Stain

[Jessica Riggleman]

RBS is the same as Methylene Blue, I believe.



_____________________________________________________________________

Jessica Riggleman | Research Associate

Globus Medical, Inc.
Valley Forge Business Center
2560 General Armistead Avenue | Audubon, PA 19403
Ph: (610) 930-1800 ext. 2583 | Fax:

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-----Original Message-----
From: Alicia Marie Ortega [mailto:alicia.ortega at colorado.edu]
Sent: Thursday, September 22, 2016 4:50 PM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Question on Sanderson Rapid Bone Stain

Hello everyone,


I am attempting to stain 30-40 micron thick undecalcified bone sections embedded in poly(methyl methacrylate) with Sanderson Rapid Bone Stain (RBS) with a Van Geison counter stain.


My first attempt at this stain resulted in a very faint stain from the RBS (I could see very faint blue/green staining of osteoid/soft tissues) and a very intense dark pink stain from the counterstain.  I was wondering if anyone knows of a way to intensify the staining of the RBS?  I heated the stain to 55-60 degrees Celsius as recommended by the manufacturer prior to staining (with a 10 minute stain duration).


Thank you in advance for your time and help.


Sincerely,

Alicia Ortega

Postdoctoral Research Associate

University of Colorado, Boulder