[Histonet] Question on Sanderson Rapid Bone Stain

[Alicia Marie Ortega]

Hello everyone,


I am attempting to stain 30-40 micron thick undecalcified bone sections embedded in poly(methyl methacrylate) with Sanderson Rapid Bone Stain (RBS) with a Van Geison counter stain.


My first attempt at this stain resulted in a very faint stain from the RBS (I could see very faint blue/green staining of osteoid/soft tissues) and a very intense dark pink stain from the counterstain.  I was wondering if anyone knows of a way to intensify the staining of the RBS?  I heated the stain to 55-60 degrees Celsius as recommended by the manufacturer prior to staining (with a 10 minute stain duration).


Thank you in advance for your time and help.


Sincerely,

Alicia Ortega

Postdoctoral Research Associate

University of Colorado, Boulder