[Histonet] Question on Sanderson Rapid Bone Stain
Alicia Marie Ortega
alicia.ortega at colorado.edu
Thu Sep 22 15:50:28 CDT 2016
I am attempting to stain 30-40 micron thick undecalcified bone sections embedded in poly(methyl methacrylate) with Sanderson Rapid Bone Stain (RBS) with a Van Geison counter stain.
My first attempt at this stain resulted in a very faint stain from the RBS (I could see very faint blue/green staining of osteoid/soft tissues) and a very intense dark pink stain from the counterstain. I was wondering if anyone knows of a way to intensify the staining of the RBS? I heated the stain to 55-60 degrees Celsius as recommended by the manufacturer prior to staining (with a 10 minute stain duration).
Thank you in advance for your time and help.
Postdoctoral Research Associate
University of Colorado, Boulder
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