[Histonet] Cutting blocks for mouse tissue troubleshooting

Elizabeth Chlipala liz at premierlab.com
Thu Oct 6 10:29:31 CDT 2016


If your samples are fixed and processed properly the sections should cut without wrinkles.  We process mouse pancreas on a 30 minute cycle - 30 minutes per station starting at 50% alcohol.  We trim and soak blocks on ice and we normally do not have issue with wrinkles.


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
liz at premierlab.com

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Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504

-----Original Message-----
From: Blanca Lopez via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Wednesday, October 05, 2016 8:06 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Cutting blocks for mouse tissue troubleshooting

Hello Histonets!
I  need to find out a better way to cut the whole pancreas mouse tissue without wrinkles, Does anybody has suggestion. I am having trouble to obtain a nice section without wrinkles. It is pretty big piece and crawl in on my embedding, is hard to stretch out without breaking. I cannot use ammonia water because they use the unstained for research and I don't want get discrepancies in any other stains.  Suggestions???

Blanca Lopez
Histotech (ASCP)
UTSW Tissue Resource K1.210
Simmons Comprehensive Cancer Center
UT Southwestern Medical Center
Telephone: 214-648-7598
Email: Blanca.Lopez at utsouthwestern.edu


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